Kawamoto S, Nozaki C, Tanaka A, Fukui S
Eur J Biochem. 1978 Feb;83(2):609-13. doi: 10.1111/j.1432-1033.1978.tb12130.x.
Localization of fatty acid beta-oxidation system in microbodies of Candida tropicalis cells growing on n-alkanes was studied. Microbodies isolated from the yeast cells showed palmitate-dependent activities of NAD reduction, acetyl-CoA formation and oxygen consumption. When sodium azide, an inhibitor of catalase, was added to the system, palmitate-dependent formation of hydrogen peroxide was observed. Stoichiometric study revealed that two moles of NAD were reduced per one mole of oxygen consumed in the absence of sodium azide and the presence of the inhibitor doubled the oxygen consumption by microbodies without an appreciable change in NAD reduction. These results indicate that the yeast microbodies contain beta-oxidation system of fatty acid, and that catalase located in the organelles participates in the degradation of hydrogen peroxide to be formed at the step of dehydrogenation of acyl-CoA.
研究了热带假丝酵母细胞在正构烷烃上生长时脂肪酸β-氧化系统在微体中的定位。从酵母细胞中分离出的微体显示出棕榈酸酯依赖的NAD还原、乙酰辅酶A形成和氧气消耗活性。当向系统中添加过氧化氢酶抑制剂叠氮化钠时，观察到棕榈酸酯依赖的过氧化氢形成。化学计量学研究表明，在没有叠氮化钠的情况下，每消耗一摩尔氧气，有两摩尔NAD被还原，并且该抑制剂使微体的氧气消耗量增加了一倍，而NAD还原没有明显变化。这些结果表明，酵母微体含有脂肪酸β-氧化系统，并且位于细胞器中的过氧化氢酶参与了在酰基辅酶A脱氢步骤中形成的过氧化氢的降解。
