Osumi M, Fukuzumi F, Teranishi Y, Tanaka A, Fukui F
Arch Microbiol. 1975 Mar 12;103(1):I-II. doi: 10.1007/BF00436323.
Development of microbodies in Candida tropicalis pK 233 was studied mainly by electron microscopical observation. The yeast cells, precultured on malt extract, scarcely contained microbodies and showed very low catalase activity. When the precultured cells were transferred to a n-alkane medium and incubated with shaking, the number of microbodies increased and concomitantly the activity of catalase was enhanced. That is, both the area ratio of microbodies in the cell and the ratio of microbodies to cytoplasm in area increased significantly during the utilization of n-alkanes for 8 hrs. Localization of catalase in the microbodies was demonstrated cytochemically by use of 3,3'-diaminobenzidine, but other organella in the cell, except for vacuoles appearing in the early growth phase and mitochondria, were not stained with this reagent. Microbodies seemed to grow by division. Biogenesis of microbodies in the yeast cells is also discussed.
主要通过电子显微镜观察研究了热带假丝酵母pK 233中微体的发育情况。在麦芽提取物上预培养的酵母细胞几乎不含微体，过氧化氢酶活性也很低。当将预培养的细胞转移到正构烷烃培养基中并振荡培养时，微体数量增加，同时过氧化氢酶活性增强。也就是说，在利用正构烷烃8小时的过程中，细胞中微体的面积比以及微体与细胞质的面积比均显著增加。通过使用3,3'-二氨基联苯胺进行细胞化学定位，证明过氧化氢酶存在于微体中，但细胞中的其他细胞器，除了在生长早期出现的液泡和线粒体之外，均未被该试剂染色。微体似乎通过分裂生长。本文还讨论了酵母细胞中微体的生物发生。
