The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Shandong University, PR China.
Biochem Biophys Res Commun. 2010 Jun 4;396(3):691-5. doi: 10.1016/j.bbrc.2010.04.163. Epub 2010 May 6.
In this study, we examined whether matrine could inhibit the differentiation of 3T3-L1 preadipocytes and further explored the possible inhibitory mechanisms. Evidenced by Oil Red O staining and AdipoRed assay, matrine dose-dependently inhibited lipid accumulation at concentrations of 125, 250 and 500 microg/ml. At molecular level, the expression of transcription factors, PPARgamma and C/EBPalpha, was reduced by matrine during adipogenesis. After treatment for 6 days, the mRNA levels of adipocyte-specific genes, such as aP2, LPL, adiponectin and leptin, were also down-regulated by matrine in a dose-dependent manner. Moreover, 500 microg/ml matrine inhibited the phosphorylation of ERK1/2 at the early stage of differentiation. Our results indicate that inhibition of 3T3-L1 preadipocyte differentiation by matrine is associated with the suppression of ERK1/2 phosphorylation. Thus, matrine has the potential to be an alternative natural product for the treatment of obesity.
在这项研究中，我们研究了苦参碱是否能抑制 3T3-L1 前脂肪细胞的分化，并进一步探讨了可能的抑制机制。油红 O 染色和 AdipoRed 检测结果表明，苦参碱在 125、250 和 500μg/ml 浓度下呈剂量依赖性抑制脂肪积累。在分子水平上，苦参碱在脂肪生成过程中降低了转录因子 PPARγ和 C/EBPα的表达。苦参碱处理 6 天后，脂肪细胞特异性基因如 aP2、LPL、脂联素和瘦素的 mRNA 水平也呈剂量依赖性下调。此外，500μg/ml 苦参碱抑制了分化早期 ERK1/2 的磷酸化。我们的结果表明，苦参碱抑制 3T3-L1 前脂肪细胞分化与抑制 ERK1/2 磷酸化有关。因此，苦参碱有可能成为治疗肥胖的替代天然产物。
