Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48 Str., 60-627 Poznan, Poland.
Food Chem. 2015 Oct 15;185:383-8. doi: 10.1016/j.foodchem.2015.03.152. Epub 2015 Apr 9.
It has previously been shown that lyophilized cranberries (LCB) decreased lipid accumulation in 3T3-L1 cells and inhibited preadipocyte differentiation by down-regulation of the expression of key transcription factors (PPARγ, C/EBPα, SREBP1) of the adipogenesis pathway. To elucidate the molecular basis of anti-lipogenic activity of LCB, the expression of several genes involved in lipid metabolism, such as adipocyte fatty acid-binding protein (aP2), lipoprotein lipase (LPL), fatty acid synthase (FAS), hormone sensitive lipase (HSL) and perilipin 1 (PLIN1), was examined in the present study. Additionally, the effects of LCB on adiponectin and leptin expression and protein secretion were also investigated. LCB reduced lipid accumulation during preadipocyte differentiation by down-regulation of the mRNA level of aP2, FAS, LPL, HSL and PLIN1. Moreover, LCB decreased leptin gene expression and increased adiponectin gene expression and protein secretion in a dose-dependent manner. Therefore cranberries could be considered as bioactive factors, which are effective in the inhibition of adipose tissue mass production.
先前的研究表明，冻干蔓越莓（LCB）通过下调脂肪生成途径中的关键转录因子（PPARγ、C/EBPα、SREBP1）的表达，减少 3T3-L1 细胞中的脂质积累并抑制前脂肪细胞分化。为了阐明 LCB 抗脂肪生成活性的分子基础，本研究检测了参与脂质代谢的几个基因的表达，如脂肪细胞脂肪酸结合蛋白（aP2）、脂蛋白脂肪酶（LPL）、脂肪酸合酶（FAS）、激素敏感脂肪酶（HSL）和脂滴包被蛋白 1（PLIN1）。此外，还研究了 LCB 对脂联素和瘦素表达和蛋白分泌的影响。LCB 通过下调 aP2、FAS、LPL、HSL 和 PLIN1 的 mRNA 水平，减少前脂肪细胞分化过程中的脂质积累。此外，LCB 呈剂量依赖性地降低瘦素基因表达，增加脂联素基因表达和蛋白分泌。因此，蔓越莓可以被认为是生物活性因子，可有效抑制脂肪组织质量的产生。
