School of Women's and Infants' Health, University of Western Australia, Perth, WA, Australia.
Am J Obstet Gynecol. 2010 May;202(5):471.e1-11. doi: 10.1016/j.ajog.2010.02.031.
The purpose of this study was to test the hypothesis that periodontopathic bacteria exert potent proinflammatory effects in human decidua.
The immunostimulatory effects of Gram-positive and negative periodontopathic bacteria and their lipopolysaccharides were tested in human decidual cell cultures in comparison with Escherichia coli. Cytokine production was measured by enzyme-linked immunosorbent assay; inflammatory gene expression was measured by oligonucleotide arrays and quantitative real time-polymerase chain reaction.
All bacteria that were tested elicited an inflammatory response, although concentration-dependence and efficacy varied considerably with organism and culture. Lipopolysaccharides were more potent stimuli than intact bacterial cells, although bacteria exerted greater effects at high concentrations. Of 112 genes on the arrays, 18 genes were stimulated significantly by one or more lipopolysaccharide preparation.
The ability of periodontopathic bacteria to stimulate a decidual inflammatory response is highly variable and partly dependent on the presence and structure of constituent lipopolysaccharides. This adds to our understanding of the causal association between periodontal disease and preterm birth.
本研究旨在验证牙周病细菌在人蜕膜中发挥强烈促炎作用的假设。
将革兰氏阳性和阴性牙周病细菌及其脂多糖与大肠杆菌进行比较，在人蜕膜细胞培养物中测试其免疫刺激作用。通过酶联免疫吸附试验测量细胞因子的产生；通过寡核苷酸阵列和实时定量聚合酶链反应测量炎症基因的表达。
所有测试的细菌都引发了炎症反应，尽管浓度依赖性和效果因生物体和培养物而异。脂多糖比完整的细菌细胞更具刺激作用，尽管细菌在高浓度下会产生更大的影响。在 112 个基因阵列中，有 18 个基因被一种或多种脂多糖制剂显著刺激。
牙周病细菌刺激蜕膜炎症反应的能力具有高度可变性，部分取决于组成脂多糖的存在和结构。这增加了我们对牙周病和早产之间因果关系的理解。
