Mitchell M D, Rice G E, Vaswani K, Kvaskoff D, Peiris H N
University of Queensland Centre for Clinical Research, Centre for Clinical Diagnostics, University of Queensland, Royal Brisbane and Women's Hospital, Queensland, Brisbane, Australia.
PLoS One. 2016 Feb 3;11(2):e0148306. doi: 10.1371/journal.pone.0148306. eCollection 2016.
An increase in intrauterine prostaglandin production is critical for the onset and progression of labor in women and indeed all mammalian species studied. Endocannabinoids can act as substrates for enzymes of the prostaglandin biosynthetic pathways and can be utilized to generate other related compounds such as prostamides. The end products are indistinguishable by radioimmunoassay. We have separated such compounds by mass spectrometry. We now show that inflammatory stimuli such as LPS and proinflammatory cytokines act differentially on these pathways in human choriodecidua and preferentially create drive through to prostaglandin end products. These findings create doubt about the interpretation of data on prostaglandin biosynthesis in intrauterine tissues from pregnant women especially in the presence of an infection. The possibility is raised that separation of these products might reduce variability in results and lead to potential uses for their measurement in the diagnosis of preterm labor.
子宫内前列腺素生成的增加对于人类以及所有已研究的哺乳动物物种分娩的发动和进展至关重要。内源性大麻素可作为前列腺素生物合成途径中酶的底物，并可用于生成其他相关化合物，如前列腺酰胺。这些终产物通过放射免疫测定法无法区分。我们已通过质谱法分离出此类化合物。我们现在表明，诸如脂多糖和促炎细胞因子等炎症刺激对人绒毛膜蜕膜中的这些途径有不同作用，并优先驱动生成前列腺素终产物。这些发现使人对孕妇子宫内组织中前列腺素生物合成数据的解读产生怀疑，尤其是在存在感染的情况下。有人提出，分离这些产物可能会降低结果的变异性，并使其在早产诊断中的测量具有潜在用途。
