在 Mycobacterium neoaurum NwIB-01 中主要的 3-酮类固醇-{Delta}1-脱氢酶的失活和增强：大豆植物甾醇向 4-雄烯-3,17-二酮或 1,4-雄二烯-3,17-二酮的生物转化。
Inactivation and augmentation of the primary 3-ketosteroid-{delta}1- dehydrogenase in Mycobacterium neoaurum NwIB-01: biotransformation of soybean phytosterols to 4-androstene- 3,17-dione or 1,4-androstadiene-3,17-dione.
机构信息
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
出版信息
Appl Environ Microbiol. 2010 Jul;76(13):4578-82. doi: 10.1128/AEM.00448-10. Epub 2010 May 7.
Abstract
3-Ketosteroid-Delta(1)-dehydrogenase, KsdD(M), was identified by targeted gene disruption and augmentation from Mycobacterium neoaurum NwIB-01, a newly isolated strain. The difficulty of separating 4-androstene-3,17-dione (AD) from 1,4-androstadiene-3,17-dione (ADD) is a key bottleneck to the microbial transformation of phytosterols in industry. This problem was tackled via genetic manipulation of the KsdD-encoding gene. Mutants in which KsdD(M) was inactivated or augmented proved to be good AD(D)-producing strains.
摘要
3-酮类固醇-Δ(1)-脱氢酶（KsdD(M)）是通过靶向基因敲除和扩增从一株新分离的分枝杆菌 NwIB-01 中鉴定得到的。从植物甾醇中微生物转化分离 4-雄烯-3,17-二酮（AD）和 1,4-雄二烯-3,17-二酮（ADD）的困难是工业上的一个关键瓶颈。通过对 KsdD 编码基因的遗传操作解决了这个问题。证明失活或扩增 KsdD(M)的突变体能很好地生产 AD(D)。
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