Jung Bock-Gie, Ko Jae-Hyung, Cho Sun-Ju, Koh Hong-Bum, Yoon So-Rah, Han Dong-Un, Lee Bong-Joo
College of Veterinary Medicine, Chonnam National University, buk-gu, Gwangju, Republic of Korea.
J Vet Med Sci. 2010 Sep;72(9):1195-202. doi: 10.1292/jvms.09-0555. Epub 2010 Apr 28.
Maesil (Prunus mume) has long been used as a traditional drug and healthy food in East Asian countries. It possesses a number of beneficial biological activities including potential antimicrobial effects against pathogens. Probiotics also have antibacterial effects. Moreover, some probiotics have an important role in regulating the immune system. The present study evaluated the immune enhancing effects of fermented Maesil with probiotics (Saccharomyces cerevisiae, Bacillus subtilis and Lactobacillus acidophilus) in mice, especially against Bordetella bronchiseptica, as an initial step towards the development of feed supplements for the promotion of immune activity and prevention of disease, especially in pigs. Continuous ingestion of fermented Maesil with probiotics markedly increased the macrophage ratio in peripheral blood and the T lymphocyte ratio in the spleen. In addition, antibody production against formalin-killed B. bronchiseptica significantly increased in the mice fed fermented Maesil compared with the control group. The number of leukocytes was significantly higher in the bronchio-alveolar lavage obtained from the fermented Maesil-fed animals compared to it in the control group at day 3 (maximal peak time) after experimental B. bronchiseptica infection. Moreover, at 7 day post-infection, relative messenger RNA expression levels of tumor necrosis factor- α and interferon-γ were significantly increased in splenocytes of mice fed fermented Maesil compared with those in the control group. Taken together, these findings suggest that feed containing fermented Maesil with probiotics enhances immune activity in mice, especially against B. bronchiseptica, via the potent stimulation of non-specific immune responses.
青梅（Prunus mume）长期以来在东亚国家被用作传统药物和健康食品。它具有许多有益的生物活性，包括对病原体的潜在抗菌作用。益生菌也有抗菌作用。此外，一些益生菌在调节免疫系统方面发挥着重要作用。本研究评估了益生菌（酿酒酵母、枯草芽孢杆菌和嗜酸乳杆菌）发酵青梅对小鼠的免疫增强作用，特别是对支气管败血波氏杆菌的作用，这是开发促进免疫活性和预防疾病的饲料补充剂的第一步，尤其是针对猪。持续摄入益生菌发酵青梅显著提高了外周血中的巨噬细胞比例和脾脏中的T淋巴细胞比例。此外，与对照组相比，喂食发酵青梅的小鼠体内针对福尔马林灭活的支气管败血波氏杆菌的抗体产生显著增加。在实验性支气管败血波氏杆菌感染后第3天（最大峰值时间），喂食发酵青梅的动物支气管肺泡灌洗中的白细胞数量显著高于对照组。此外，在感染后7天，喂食发酵青梅的小鼠脾细胞中肿瘤坏死因子-α和干扰素-γ的相对信使核糖核酸表达水平显著高于对照组。综上所述，这些发现表明，含有益生菌发酵青梅的饲料通过有力刺激非特异性免疫反应增强了小鼠的免疫活性，特别是针对支气管败血波氏杆菌的免疫活性。
