Pilione Mylisa R, Harvill Eric T
Department of Veterinary and Biomedical Science, The Pennsylvania State University, 115 Henning, University Park, PA 16802, USA.
Infect Immun. 2006 Feb;74(2):1043-9. doi: 10.1128/IAI.74.2.1043-1049.2006.
Several species of pathogenic microorganisms have developed strategies to survive and persist in vital organs which are normally maintained as sterile by the generation of strong immune responses. Here, we report an immunomodulation involving the Bordetella bronchiseptica type III secretion system (TTSS) which contributes to bacterial survival in the lower respiratory tract of the host. The prolonged persistence of B. bronchiseptica that was observed in gamma interferon (IFN-gamma)-/- mice indicates that the efficient clearance of bacteria from the lower respiratory tract requires not only B cells and antibodies but also IFN-gamma production. Our data also suggest that interleukin-10 (IL-10)-producing splenocytes are generated early during infection and that IL-10 inhibits IFN-gamma-producing cells and delays the clearance of B. bronchiseptica from the lungs. The TTSS of B. bronchiseptica inhibits the generation of IFN-gamma-producing splenocytes and is required for long-term bacterial persistence in the lower respiratory tract in wild-type mice. This suggests that a mechanism involving the modulation of IFN-gamma production by the TTSS facilitates B. bronchiseptica survival in the lower respiratory tract.
几种致病微生物已经形成了在重要器官中存活和持续存在的策略，而这些重要器官通常通过产生强烈的免疫反应来保持无菌状态。在此，我们报告了一种涉及支气管败血博德特氏菌III型分泌系统（TTSS）的免疫调节作用，该系统有助于细菌在宿主下呼吸道中存活。在γ干扰素（IFN-γ）基因敲除小鼠中观察到支气管败血博德特氏菌的长期持续存在，这表明从下呼吸道有效清除细菌不仅需要B细胞和抗体，还需要产生IFN-γ。我们的数据还表明，在感染早期会产生产生白细胞介素10（IL-10）的脾细胞，并且IL-10会抑制产生IFN-γ的细胞，并延迟支气管败血博德特氏菌从肺部的清除。支气管败血博德特氏菌的TTSS会抑制产生IFN-γ的脾细胞的生成，并且是野生型小鼠下呼吸道中细菌长期持续存在所必需的。这表明一种涉及TTSS调节IFN-γ产生的机制促进了支气管败血博德特氏菌在下呼吸道中的存活。
