• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

deaD是大肠杆菌中一个新的基因，编码一种推测的ATP依赖性RNA解旋酶，它能够抑制rpsB（编码核糖体蛋白S2的基因）中的一个突变。

deaD, a new Escherichia coli gene encoding a presumed ATP-dependent RNA helicase, can suppress a mutation in rpsB, the gene encoding ribosomal protein S2.

作者信息

Toone W M, Rudd K E, Friesen J D

机构信息

Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.

出版信息

J Bacteriol. 1991 Jun;173(11):3291-302. doi: 10.1128/jb.173.11.3291-3302.1991.

DOI:10.1128/jb.173.11.3291-3302.1991
PMID:2045359
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207940/
Abstract

We have cloned and sequenced a new gene from Escherichia coli which encodes a 64-kDa protein. The inferred amino acid sequence of the protein shows remarkable similarity to eIF4A, a murine translation initiation factor that has an ATP-dependent RNA helicase activity and is a founding member of the D-E-A-D family of proteins (characterized by a conserved Asp-Glu-Ala-Asp motif). Our new gene, called deaD, was cloned as a gene dosage-dependent suppressor of temperature-sensitive mutations in rpsB, the gene encoding ribosomal protein S2. We suggest that the DeaD protein plays a hitherto unknown role in translation in E. coli.

摘要

我们从大肠杆菌中克隆并测序了一个新基因，该基因编码一种64 kDa的蛋白质。该蛋白质的推断氨基酸序列与eIF4A具有显著相似性，eIF4A是一种小鼠翻译起始因子，具有ATP依赖性RNA解旋酶活性，是D-E-A-D蛋白家族的创始成员（其特征是具有保守的天冬氨酸-谷氨酸-丙氨酸-天冬氨酸基序）。我们的新基因称为deaD，它是作为核糖体蛋白S2编码基因rpsB中温度敏感突变的基因剂量依赖性抑制子而克隆的。我们认为DeaD蛋白在大肠杆菌的翻译过程中发挥着迄今未知的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c85/207940/093e424db5e8/jbacter00101-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c85/207940/ec0dae21f6ac/jbacter00101-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c85/207940/093e424db5e8/jbacter00101-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c85/207940/ec0dae21f6ac/jbacter00101-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c85/207940/093e424db5e8/jbacter00101-0035-a.jpg

相似文献

1
deaD, a new Escherichia coli gene encoding a presumed ATP-dependent RNA helicase, can suppress a mutation in rpsB, the gene encoding ribosomal protein S2.deaD是大肠杆菌中一个新的基因，编码一种推测的ATP依赖性RNA解旋酶，它能够抑制rpsB（编码核糖体蛋白S2的基因）中的一个突变。
J Bacteriol. 1991 Jun;173(11):3291-302. doi: 10.1128/jb.173.11.3291-3302.1991.
2
Cloning and characterization of the hrpA gene in the terC region of Escherichia coli that is highly similar to the DEAH family RNA helicase genes of Saccharomyces cerevisiae.大肠杆菌terC区域中hrpA基因的克隆与特性分析，该基因与酿酒酵母的DEAH家族RNA解旋酶基因高度相似。
Nucleic Acids Res. 1995 Feb 25;23(4):595-8. doi: 10.1093/nar/23.4.595.
3
Effects of ribosomal proteins S1, S2 and the DeaD/CsdA DEAD-box helicase on translation of leaderless and canonical mRNAs in Escherichia coli.核糖体蛋白S1、S2以及DeaD/CsdA DEAD盒解旋酶对大肠杆菌中无起始密码子mRNA和典型mRNA翻译的影响。
Mol Microbiol. 2002 Jun;44(5):1387-96. doi: 10.1046/j.1365-2958.2002.02971.x.
4
rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote.rhlB是大肠杆菌K-12中的一个新基因，其具有类RNA解旋酶蛋白序列基序，是原核生物中至少五个此类可能基因之一。
New Biol. 1991 Sep;3(9):886-95.
5
The embryonic RNA helicase gene (ERH): a new member of the DEAD box family of RNA helicases.
Biochem J. 1995 Jun 15;308 ( Pt 3)(Pt 3):839-46. doi: 10.1042/bj3080839.
6
Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts.大肠杆菌中一个新的核糖体操纵子的组织和核苷酸序列，该操纵子包含核糖体蛋白S2和延伸因子Ts的基因。
Nucleic Acids Res. 1981 Aug 25;9(16):4163-72. doi: 10.1093/nar/9.16.4163.
7
Translation initiation and ribosomal biogenesis: involvement of a putative rRNA helicase and RPL46.翻译起始与核糖体生物发生：一种假定的rRNA解旋酶和RPL46的参与
Science. 1990 Mar 2;247(4946):1077-9. doi: 10.1126/science.2408148.
8
The molecular cloning of the gene encoding the Escherichia coli 75-kDa helicase and the determination of its nucleotide sequence and gentic map position.编码大肠杆菌75-kDa解旋酶的基因的分子克隆及其核苷酸序列和遗传图谱位置的确定。
J Biol Chem. 1989 May 15;264(14):8297-303.
9
Organization and nucleotide sequence of the genes for ribosomal protein S2 and elongation factor Ts in Spirulina platensis.钝顶螺旋藻核糖体蛋白S2和延伸因子Ts基因的组织与核苷酸序列
FEMS Microbiol Lett. 1990 Jan 1;54(1-3):141-5. doi: 10.1016/0378-1097(90)90272-r.
10
ATP hydrolysis by initiation factor 4A is required for translation initiation in Saccharomyces cerevisiae.酿酒酵母的翻译起始需要起始因子4A水解ATP。
Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7664-8. doi: 10.1073/pnas.89.16.7664.

引用本文的文献

1
DEAD Box RNA Helicases: Biochemical Properties, Role in RNA Processing and Ribosome Biogenesis.DEAD 盒 RNA 解旋酶：生化特性、在 RNA 加工和核糖体生物发生中的作用。
Cell Biochem Biophys. 2024 Jun;82(2):427-434. doi: 10.1007/s12013-024-01240-w. Epub 2024 Mar 2.
2
Identification of Myelin Basic Protein Proximity Interactome Using TurboID Labeling Proteomics.使用 TurboID 标记蛋白质组学鉴定髓鞘碱性蛋白邻近相互作用组。
Cells. 2023 Mar 20;12(6):944. doi: 10.3390/cells12060944.
3
Peptidoglycan Remodeling by an L,D-Transpeptidase, LdtD during Cold Shock in Escherichia coli.

本文引用的文献

1
Organization of genes in the four minute region of the Escherichia coli chromosome: evidence that rpsB and tsf are co-transcribed.大肠杆菌染色体四分钟区域内基因的组织：核糖体蛋白S2基因（rpsB）和延伸因子Tu基因（tsf）共转录的证据。
Mol Gen Genet. 1981;181(3):356-62. doi: 10.1007/BF00425611.
2
Model for regulation of the histidine operon of Salmonella.沙门氏菌组氨酸操纵子调控模型。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):508-12. doi: 10.1073/pnas.77.1.508.
3
Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
在大肠杆菌的冷休克中，L,D-转肽酶 LdtD 对肽聚糖进行重塑。
J Bacteriol. 2023 Jan 26;205(1):e0038222. doi: 10.1128/jb.00382-22. Epub 2022 Dec 12.
4
Dual-level autoregulation of the DeaD RNA helicase via mRNA stability and Rho-dependent transcription termination.通过 mRNA 稳定性和 Rho 依赖的转录终止对 DeaD RNA 解旋酶进行双重水平的自动调节。
RNA. 2020 Sep;26(9):1160-1169. doi: 10.1261/rna.074112.119. Epub 2020 May 15.
5
iTRAQ-based quantitative proteomic analysis of salt stress in Spica Prunellae.基于 iTRAQ 的夏枯草盐胁迫定量蛋白质组学分析。
Sci Rep. 2019 Jul 3;9(1):9590. doi: 10.1038/s41598-019-46043-9.
6
Proteomic Analysis of Vibrio parahaemolyticus Under Cold Stress.副溶血性弧菌在冷胁迫下的蛋白质组学分析
Curr Microbiol. 2018 Jan;75(1):20-26. doi: 10.1007/s00284-017-1345-4. Epub 2017 Aug 22.
7
Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes.链特异性RNA测序和膜脂谱分析揭示了单核细胞增生李斯特菌中依赖生长阶段的冷应激反应机制。
PLoS One. 2017 Jun 29;12(6):e0180123. doi: 10.1371/journal.pone.0180123. eCollection 2017.
8
Biosynthesis of Histidine.组氨酸的生物合成
EcoSal Plus. 2009 Aug;3(2). doi: 10.1128/ecosalplus.3.6.1.9.
9
The RNA Helicase DeaD Stimulates ExsA Translation To Promote Expression of the Pseudomonas aeruginosa Type III Secretion System.RNA解旋酶DeaD刺激ExsA翻译以促进铜绿假单胞菌Ⅲ型分泌系统的表达。
J Bacteriol. 2015 Aug;197(16):2664-74. doi: 10.1128/JB.00231-15. Epub 2015 Jun 8.
10
Characterization of the kinetics of RNA annealing and strand displacement activities of the E. coli DEAD-box helicase CsdA.解析大肠杆菌 DEAD-box 解旋酶 CsdA 的 RNA 退火和链置换活性的动力学特征。
RNA Biol. 2013 Jan;10(1):149-56. doi: 10.4161/rna.23475. Epub 2013 Jan 1.
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.
4
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。附录
Anal Biochem. 1984 Feb;137(1):266-7. doi: 10.1016/0003-2697(84)90381-6.
5
Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts.大肠杆菌中一个新的核糖体操纵子的组织和核苷酸序列，该操纵子包含核糖体蛋白S2和延伸因子Ts的基因。
Nucleic Acids Res. 1981 Aug 25;9(16):4163-72. doi: 10.1093/nar/9.16.4163.
6
Protein-DNA recognition.蛋白质-脱氧核糖核酸识别
Annu Rev Biochem. 1984;53:293-321. doi: 10.1146/annurev.bi.53.070184.001453.
7
Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.大肠杆菌rplJL - rpoBC转录单元RNA的衰减与加工
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331-5. doi: 10.1073/pnas.77.6.3331.
8
The product of gene secC is involved in the synthesis of exported proteins in E. coli.
Cell. 1984 Aug;38(1):211-7. doi: 10.1016/0092-8674(84)90542-7.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
Genetic analysis of diaminopimelic acid- and lysine-requiring mutants of Escherichia coli.大肠杆菌中对二氨基庚二酸和赖氨酸有需求的突变体的遗传分析。
J Bacteriol. 1971 Mar;105(3):844-54. doi: 10.1128/jb.105.3.844-854.1971.