Sachs A B, Davis R W
Department of Biochemistry, Stanford Medical Center, CA 94305.
Science. 1990 Mar 2;247(4946):1077-9. doi: 10.1126/science.2408148.
Cold-sensitive mutations in the SPB genes (spb1-spb7) of Saccharomyces cerevisiae suppress the inhibition of translation initiation resulting from deletion of the poly(A)-binding protein gene (PAB1). The SPB4 protein belongs to a family of adenosine triphosphate (ATP)-dependent RNA helicases. The aberrant production of 25S ribosomal RNA (rRNA) occurring in spb4-1 mutants or the deletion of SPB2 (RPL46) permits the deletion of PAB1. These data suggest that mutations affecting different steps of 60S subunit formation can allow PAB-independent translation, and they indicate that further characterization of the spb mutations could lend insight into the biogenesis of the ribosome.
酿酒酵母SPB基因（spb1 - spb7）中的冷敏感突变可抑制因多聚腺苷酸结合蛋白基因（PAB1）缺失而导致的翻译起始抑制。SPB4蛋白属于依赖三磷酸腺苷（ATP）的RNA解旋酶家族。spb4 - 1突变体中出现的25S核糖体RNA（rRNA）异常产生或SPB2（RPL46）的缺失允许PAB1缺失。这些数据表明，影响60S亚基形成不同步骤的突变可允许不依赖PAB的翻译，并且表明对spb突变的进一步表征可能有助于深入了解核糖体的生物发生。
