Microbial Ecology Laboratory, Aquaculture Department, Faculty of Marine Resources, University of Antofagasta, Casilla 170, Antofagasta, Chile.
Microb Ecol. 2010 Oct;60(3):599-605. doi: 10.1007/s00248-010-9674-x. Epub 2010 May 9.
The pandemic bacterium Vibrio parahaemolyticus, isolated from seawater, sediment, and marine organisms, is responsible for gastroenteric illnesses in humans and also cause diseases in aquaculture industry in Chile and other countries around the world. In this study, bacterial flora with inhibitory activity against pathogenic V. parahaemolyticus were collected from egg capsules of Concholepas concholepas and evaluated. The 16S rRNA fragment was sequenced from each isolated strain to determine its identity using the GenBank database. A phylogenetic analysis was made, and tests for the productions of antibacterial substance were performed using the double-layer method. Forty-five morphotypes of bacterial colonies were isolated, 8 of which presented an inhibitory effect on the growth of V. parahaemolyticus. 16S rRNA sequence and phylogenetic analysis show that these strains constitute taxa that are phylogenetically related to the Bacillus genus and are probably sister species or strains of the species Bacillus pumilus, Bacillus licheniform, or Bacillus sp. It is important to determine the nature of the antibacterial substance to evaluate their potential for use against the pathogen species V. parahaemolyticus.
从海水、沉积物和海洋生物中分离出的弧菌属病原菌副溶血性弧菌可引起人类肠胃疾病，也可导致智利和世界其他国家水产养殖业的疾病。本研究从糙海参(Congcholepas concholepas)的卵囊中收集了具有抑制致病性副溶血性弧菌活性的细菌菌群，并对其进行了评估。从每个分离株中提取 16S rRNA 片段，并使用 GenBank 数据库确定其身份。进行了系统发育分析，并使用双层法进行了抗菌物质产生的测试。从细菌菌落中分离出 45 种形态，其中 8 种对副溶血性弧菌的生长有抑制作用。16S rRNA 序列和系统发育分析表明，这些菌株构成与芽孢杆菌属亲缘关系密切的分类群，可能是芽孢杆菌属的姐妹种或芽孢杆菌属的菌株，如短小芽孢杆菌、地衣芽孢杆菌或芽孢杆菌属。确定抗菌物质的性质对于评估其对副溶血性弧菌等病原体的潜在用途非常重要。
