Lottiidibacillus patelloidae gen. nov., sp. nov., isolated from the intestinal tract of a marine limpet and reclassification of Bacillus taeanensis as Maribacillus taeanensis gen. nov., comb. nov.

A taxonomic study was carried out on strain SA5d-4, which was isolated from a marine limpet (Patelloida saccharina lanx [Reeve, 1855]) collected from intertidal rocks in Xiamen, China. Strain SA5d-4 was aerobic, Gram-positive, lacked flagellum, non-motile, filamentous, formed a slightly-yellowish colony, and non-sporulating. The strain grew optimally at 28 °C, at pH values 7.0-8.0, and in the presence of 1-2% (w/v) sodium chloride. The major cellular fatty acids identified were iso-C, iso-Cω10c, and iso-C. The dominated respiratory quinone was menaquinone-7. The major phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The genomic DNA G + C content was 35.3 mol%, calculated from a draft genome sequence. Phylogenetic analysis based on the full-length 16S rRNA gene sequence showed that strain SA5d-4 belongs to a new genus within the family Bacillaceae, and this gene shares 95.6% similarity with that from Bacillus taeanensis BH030017, 95.2% with Bacillus algicola KMM 3737, 95.1% with Bacillus alkalinitrilicus ANL-iso4, 94.9% with Bacillus hwajinpoensis SW-72, and 94.6% with Anaerobacillus alkalidiazotrophicus MS6. Whole genome phylogenetic analyses indicated that strain SA5d-4 formed a monophyletic branch with B. taeanensis BH030017. The average nucleotide identity between strain SA5d-4 and B. taeanensis BH030017 was 69.6%. Based on polyphasic taxonomic characteristics, strain SA5d-4 represents a novel species of a new genus, for which the name Lottiidibacillus patelloidae gen. nov., sp. nov., is proposed with the type strain SA5d-4 (= MCCC 1A11654 = KCTC 33831). Based on phylogenetic analyses, B. taeanensis should be transferred to a new genus, named Maribacillus, as Maribacillus taeanensis comb. nov., with type strain BH030017 (= KCTC 3918 = DSM 16466).