Liu Renju, Huang Zhaobin, Dong Chunming, Shao Zongze
State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, State Oceanic Administration; Fujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen, 361005, China.
Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, China.
Antonie Van Leeuwenhoek. 2019 May;112(5):797-807. doi: 10.1007/s10482-018-01213-z. Epub 2019 Jan 1.
A taxonomic study was carried out on strain SA5d-4, which was isolated from a marine limpet (Patelloida saccharina lanx [Reeve, 1855]) collected from intertidal rocks in Xiamen, China. Strain SA5d-4 was aerobic, Gram-positive, lacked flagellum, non-motile, filamentous, formed a slightly-yellowish colony, and non-sporulating. The strain grew optimally at 28 °C, at pH values 7.0-8.0, and in the presence of 1-2% (w/v) sodium chloride. The major cellular fatty acids identified were iso-C, iso-Cω10c, and iso-C. The dominated respiratory quinone was menaquinone-7. The major phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The genomic DNA G + C content was 35.3 mol%, calculated from a draft genome sequence. Phylogenetic analysis based on the full-length 16S rRNA gene sequence showed that strain SA5d-4 belongs to a new genus within the family Bacillaceae, and this gene shares 95.6% similarity with that from Bacillus taeanensis BH030017, 95.2% with Bacillus algicola KMM 3737, 95.1% with Bacillus alkalinitrilicus ANL-iso4, 94.9% with Bacillus hwajinpoensis SW-72, and 94.6% with Anaerobacillus alkalidiazotrophicus MS6. Whole genome phylogenetic analyses indicated that strain SA5d-4 formed a monophyletic branch with B. taeanensis BH030017. The average nucleotide identity between strain SA5d-4 and B. taeanensis BH030017 was 69.6%. Based on polyphasic taxonomic characteristics, strain SA5d-4 represents a novel species of a new genus, for which the name Lottiidibacillus patelloidae gen. nov., sp. nov., is proposed with the type strain SA5d-4 (= MCCC 1A11654 = KCTC 33831). Based on phylogenetic analyses, B. taeanensis should be transferred to a new genus, named Maribacillus, as Maribacillus taeanensis comb. nov., with type strain BH030017 (= KCTC 3918 = DSM 16466).
对菌株SA5d - 4进行了分类学研究，该菌株是从中国厦门潮间带岩石采集的海洋帽贝（Patelloida saccharina lanx [Reeve, 1855]）中分离得到的。菌株SA5d - 4为需氧菌，革兰氏阳性，无鞭毛，不运动，丝状，形成微黄色菌落，不产芽孢。该菌株在28°C、pH值7.0 - 8.0以及1 - 2%（w/v）氯化钠存在的条件下生长最佳。鉴定出的主要细胞脂肪酸为异C、异Cω10c和异C。主要呼吸醌为甲基萘醌 - 7。鉴定出的主要磷脂为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。根据基因组草图序列计算，基因组DNA的G + C含量为35.3 mol%。基于全长16S rRNA基因序列的系统发育分析表明，菌株SA5d - 4属于芽孢杆菌科内的一个新属，该基因与来自泰安芽孢杆菌BH030017的基因相似度为95.6%，与嗜藻芽孢杆菌KMM 3737的相似度为95.2%，与嗜碱腈芽孢杆菌ANL - iso4的相似度为95.1%，与华津浦芽孢杆菌SW - 72的相似度为94.9%，与嗜碱固氮厌氧芽孢杆菌MS6的相似度为94.6%。全基因组系统发育分析表明，菌株SA5d - 4与泰安芽孢杆菌BH030017形成一个单系分支。菌株SA5d - 4与泰安芽孢杆菌BH030017之间的平均核苷酸同一性为69.6%。基于多相分类学特征，菌株SA5d - 4代表一个新属的新物种，为此提出新属名为Lottiidibacillus，新种名为patelloidae gen. nov., sp. nov.，模式菌株为SA5d - 4（= MCCC 1A11654 = KCTC 33831）。基于系统发育分析，泰安芽孢杆菌应转移到一个新属，命名为Maribacillus，即泰安海芽孢杆菌Maribacillus taeanensis comb. nov.，模式菌株为BH030017（= KCTC 3918 = DSM 16466）。
