Department of Pathology, The University of Texas Medical Branch, Galveston, Texas 77555–0609, USA.
Vector Borne Zoonotic Dis. 2010 Aug;10(6):621-8. doi: 10.1089/vbz.2009.0156.
The purpose of this study was to assess the effect of inoculum dose on the pathogenesis of Black Creek Canal virus (BCCV) infection in the hispid cotton rat (Sigmodon hispidus), the principal host of BCCV. No sign of illness was observed in any of the 52 juvenile hispid cotton rats inoculated with 3.1, 1.1, -0.9, or -2.9 log(10) median infectious doses(VeroE6) (ID(50-VeroE6)) of BCCV and euthanized on day 9, 18, 27, or 54 postinoculation (PI). Analysis of virus assay and serological data indicated that inoculum dose could significantly affect the pathogenesis of BCCV infection in juvenile hispid cotton rats. For example, the six animals inoculated with 3.1 or 1.1 log(10) ID(50-VeroE6) and euthanized on day 54 PI were virus positive and antibody positive, whereas the six animals inoculated with -0.9 or -2.9 log(10) ID(50-VeroE6) and euthanized on day 54 PI were virus positive but antibody negative. Microscopic examination of tissues from the animals inoculated with 3.1 or 1.1 log(10) ID(50-VeroE6) revealed diffuse, subacute pneumonitis in the lungs of all the animals euthanized on day 18 PI or thereafter, and indicated that the severity of pneumonitis was dependent upon inoculum dose as well as duration of infection (i.e., amount of time elapsed since inoculation).
本研究旨在评估接种剂量对黑溪运河病毒（BCCV）在其主要宿主卷毛棉鼠（Sigmodon hispidus）中的发病机制的影响。52 只幼龄卷毛棉鼠接种 3.1、1.1、-0.9 或-2.9 log(10) 半数组织感染剂量（VeroE6）（ID(50-VeroE6)）的 BCCV 后，无任何疾病迹象，于接种后第 9、18、27 或 54 天安乐死。病毒检测和血清学数据分析表明，接种剂量可显著影响幼龄卷毛棉鼠 BCCV 感染的发病机制。例如，接种 3.1 或 1.1 log(10) ID(50-VeroE6)并于第 54 天安乐死的 6 只动物病毒检测阳性且抗体检测阳性，而接种-0.9 或-2.9 log(10) ID(50-VeroE6)并于第 54 天安乐死的 6 只动物病毒检测阳性但抗体检测阴性。接种 3.1 或 1.1 log(10) ID(50-VeroE6)的动物组织的显微镜检查显示，所有于第 18 天 PI 或之后安乐死的动物肺部均有弥漫性亚急性肺炎，表明肺炎的严重程度取决于接种剂量以及感染持续时间（即自接种以来的时间长短）。
