Milazzo Mary Louise, Cajimat Maria N B, Richter Martin H, Bradley Robert D, Fulhorst Charles F
1 Department of Pathology, University of Texas Medical Branch , Galveston, Texas.
2 Department of Biological Sciences, Texas Tech University , and Museum of Texas Tech University, Lubbock, Texas.
Vector Borne Zoonotic Dis. 2017 Oct;17(10):720-729. doi: 10.1089/vbz.2017.2108. Epub 2017 Jul 17.
The broad objective of this study was to increase our knowledge of Muleshoe virus and other hantaviruses associated with cricetid rodents in Texas. Anti-hantavirus antibody was found in 38 (3.2%) of 1171 neotomine rodents and 6 (1.8%) of 332 sigmodontine rodents from 10 Texas counties; hantaviral RNA was detected in 23 (71.9%) of 32 antibody-positive rodents. Analyses of nucleocapsid protein gene sequences indicated Muleshoe virus infection in four hispid cotton rats (Sigmodon hispidus) from northern Texas; Bayou virus, three Texas marsh oryzomys (Oryzomys texensis) from the Gulf Coast; Limestone Canyon virus, five brush mice (Peromyscus boylii) from western Texas; and Sin Nombre virus-five Texas mice (P. attwateri), one Lacey's white-ankled deer mouse (P. laceianus), four white-footed mice (P. leucopus), and one fulvous harvest mouse (Reithrodontomys fulvescens) from northern, central, or southern Texas. The results of this study together with the results of a previous study revealed that Muleshoe virus, perhaps in association with S. hispidus, is distributed across northern Texas. Finally, the results of Bayesian analyses of glycoprotein precursor (GPC) gene sequences and pairwise comparisons of complete GPC (amino acid) sequences strengthened support for the notion that Muleshoe virus is distinct from Black Creek Canal virus, Bayou virus, and all other species included in the Bunyaviridae, genus Hantavirus.
本研究的主要目标是增进我们对穆尔肖病毒以及与得克萨斯州仓鼠类啮齿动物相关的其他汉坦病毒的了解。在来自得克萨斯州10个县的1171只新鼠科啮齿动物中，有38只（3.2%）检测到抗汉坦病毒抗体；在332只稻鼠科啮齿动物中，有6只（1.8%）检测到该抗体。在32只抗体呈阳性的啮齿动物中，有23只（71.9%）检测到汉坦病毒RNA。核衣壳蛋白基因序列分析表明，来自得克萨斯州北部的4只刚毛棉鼠（Sigmodon hispidus）感染了穆尔肖病毒；来自墨西哥湾沿岸的3只得克萨斯稻水鼠（Oryzomys texensis）感染了河口病毒；来自得克萨斯州西部的5只灌丛鼠（Peromyscus boylii）感染了石灰岩峡谷病毒；来自得克萨斯州北部、中部或南部的5只得克萨斯小鼠（P. attwateri）、1只莱西白踝鹿鼠（P. laceianus）、4只白足鼠（P. leucopus）和1只棕褐收获鼠（Reithrodontomys fulvescens）感染了无名病毒。本研究结果与之前一项研究的结果共同表明，穆尔肖病毒可能与刚毛棉鼠有关，分布于得克萨斯州北部。最后，糖蛋白前体（GPC）基因序列的贝叶斯分析结果以及完整GPC（氨基酸）序列的成对比较结果，进一步支持了穆尔肖病毒与黑溪运河病毒、河口病毒以及汉坦病毒属布尼亚病毒科中的所有其他物种不同的观点。
