从佛罗里达州棉鼠体内分离出新型汉坦病毒——黑溪运河病毒。

Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida.

作者信息

Rollin P E, Ksiazek T G, Elliott L H, Ravkov E V, Martin M L, Morzunov S, Livingstone W, Monroe M, Glass G, Ruo S

机构信息

Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

出版信息

J Med Virol. 1995 May;46(1):35-9. doi: 10.1002/jmv.1890460108.

DOI:10.1002/jmv.1890460108

PMID:7623004

Abstract

Numerous rodents were trapped for serologic and virologic studies following the identification of a hantavirus pulmonary syndrome (HPS) case in Dade County, Florida. Cotton rats (Sigmodon hispidus) were the most frequently capture rodent and displayed the highest seroprevalence to a variety of hantavirus antigens. Hantavirus genome RNA was detected in all the seropositive cotton rats tested, using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay. A virus was isolated from tissues of two seropositive cotton rats by cultivation of lung and spleen homogenates on Vero E6 cells. Nucleotide sequence information obtained by direct RT-PCR and the serologic relationships of this virus with the other hantaviruses indicate that this virus, Black Creek Canal virus, represents a new hantavirus distinct from the previously known serotypes.

摘要

在佛罗里达州戴德县确诊一例汉坦病毒肺综合征（HPS）病例后，捕获了许多啮齿动物用于血清学和病毒学研究。棉鼠（Sigmodon hispidus）是捕获频率最高的啮齿动物，对多种汉坦病毒抗原的血清阳性率也最高。使用逆转录聚合酶链反应（RT-PCR）检测法，在所有检测的血清阳性棉鼠中均检测到汉坦病毒基因组RNA。通过在Vero E6细胞上培养肺和脾匀浆，从两只血清阳性棉鼠的组织中分离出一种病毒。通过直接RT-PCR获得的核苷酸序列信息以及该病毒与其他汉坦病毒的血清学关系表明，这种病毒，即黑溪运河病毒，代表了一种不同于先前已知血清型的新型汉坦病毒。