源自两个内源性泛素基因的新型启动子序列在转基因埃及伊蚊中的验证
Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti.
作者信息
Anderson M A E, Gross T L, Myles K M, Adelman Z N
机构信息
Fralin Life Science Institute and Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
出版信息
Insect Mol Biol. 2010 Aug;19(4):441-9. doi: 10.1111/j.1365-2583.2010.01005.x. Epub 2010 Apr 26.
Abstract
To date, only a limited number of promoter sequences have been described to drive transgene expression in the disease vector Aedes aegypti. We sought to increase this repertoire by characterizing the ability of upstream sequences derived from the Ae. aegypti Ub(L40) and polyubiquitin genes to drive the expression of marker proteins. Both genomic fragments were able to drive robust expression of luciferase in cultured mosquito cells. Following Mos1-transformation, the Ub(L40) promoter drove strong expression of a fluorescent marker in early larvae and in ovaries, while the polyubiquitin promoter drove robust EGFP expression in all stages of development, including constitutive expression throughout the midgut. These promoter fragments provide two new expression profiles for future Ae. aegypti genetic experiments.
摘要
迄今为止，仅有数量有限的启动子序列被描述用于驱动疾病载体埃及伊蚊中的转基因表达。我们试图通过表征源自埃及伊蚊Ub(L40)和多聚泛素基因的上游序列驱动标记蛋白表达的能力来增加这一序列库。这两个基因组片段均能够在培养的蚊细胞中驱动荧光素酶的强劲表达。经Mos1转化后，Ub(L40)启动子在早期幼虫和卵巢中驱动荧光标记的强表达，而多聚泛素启动子在发育的所有阶段驱动强劲的EGFP表达，包括在整个中肠中的组成型表达。这些启动子片段为未来的埃及伊蚊基因实验提供了两种新的表达模式。
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