Nanobiotix SA, F-75012 Paris, France.
J Photochem Photobiol B. 2010 Jul 2;100(1):1-9. doi: 10.1016/j.jphotobiol.2010.03.009. Epub 2010 Mar 30.
A new versatile hybrid nanocarrier has been designed using a "soft chemistry" synthesis, to efficiently encapsulate a photosensitizer - the protoporphyrin IX (Pp IX) - while preserving its activity intact in biological environment for advantageous use in photodynamic therapy (PDT). The synthesized Pp IX silica-based nanocarriers show to be spherical in shape and highly monodisperse with size extending from 10 nm up to 200 nm according to the synthesis procedure. Upon laser irradiation, the entrapped Pp IX shows to efficiently deliver reactive oxygen species (ROS) which are responsible for damaging tumor tissues. The ability of Pp IX silica-based nanocarriers to induce tumor cell death has been tested successfully in vitro. The stability of the Pp IX silica-based nanocarriers has been followed by UV-vis absorption and fluorescence emission in aqueous media and in 100% mouse serum media. The flexibility of the nanocarrier silica core has been examined as the key parameter to tune the Pp IX stability in biological environment. Indeed, an additional biocompatible inorganic surface coating performed on the Pp IX silica-based nanocarriers to produce an optimized bilayer coating demonstrates to significantly enhance the Pp IX stabilization in biological environments. Such versatile hybrid nanocarriers open new perspectives for PDT.
一种新的多功能杂化纳米载体已经通过“软化学”合成设计出来，以有效地封装光敏剂 - 原卟啉 IX（PpIX） - 同时在生物环境中保持其活性完整，有利于光动力疗法（PDT）的应用。合成的基于二氧化硅的 PpIX 纳米载体呈球形，高度单分散，尺寸根据合成工艺从 10nm 扩展到 200nm。在激光照射下，被包封的 PpIX 有效地传递活性氧（ROS），ROS 负责破坏肿瘤组织。基于 PpIX 的二氧化硅纳米载体在体外成功测试了诱导肿瘤细胞死亡的能力。通过在水介质和 100%鼠血清介质中进行紫外-可见吸收和荧光发射，跟踪了 PpIX 基于二氧化硅的纳米载体的稳定性。纳米载体二氧化硅核的灵活性已被检验为在生物环境中调整 PpIX 稳定性的关键参数。事实上，在 PpIX 基于二氧化硅的纳米载体上进行额外的生物相容的无机表面涂层以产生优化的双层涂层，证明可显著提高 PpIX 在生物环境中的稳定性。这种多功能杂化纳米载体为 PDT 开辟了新的前景。
