Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, UP 202 002, India.
Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2052-8. doi: 10.1016/j.fct.2010.05.004. Epub 2010 May 8.
Cisplatin is a widely used anticancer drug. It is documented that it elicits major side effects like nephrotoxicity and hepatotoxicity due to oxidative stress forcing the patients to limit its clinical use in long term treatment. Riboflavin (vitamin B(2)) is a strong photosensitizer because it generates reactive oxygen species (ROS) upon photoillumination. We have tried to trap its photosensitizing property to ameliorate the cisplatin induced nephrotoxicity and hepatotoxicity in mice. They were treated with riboflavin and cisplatin separately as well as with their combination under photoilluminated condition. The status of major antioxidant enzymes, antioxidant proteins, functional markers, lipid peroxidation and protein oxidation was studied in liver, kidneys and serum samples of all the groups. Cisplatin treated group showed significantly compromised level of antioxidant enzymes and the proteins with higher extent of lipid and protein oxidation. Similar but less pronounced pattern was observed in the riboflavin treated group. The groups treated with the combination of cisplatin and riboflavin showed all the parameters tended towards normal levels in a dose dependent manner. Hence, it can be hypothesized that riboflavin shows ameliorative effect on the cisplatin induced nephrotoxicity and hepatotoxicity under the mentioned treatment conditions.
顺铂是一种广泛应用的抗癌药物。有文献记载，由于氧化应激导致顺铂产生严重的副作用，如肾毒性和肝毒性，迫使患者限制其在长期治疗中的临床应用。核黄素（维生素 B(2)）是一种很强的光敏剂，因为它在光照下会产生活性氧（ROS）。我们试图利用其光敏特性来改善顺铂诱导的小鼠肾毒性和肝毒性。将核黄素和顺铂分别以及在光照条件下联合应用于这些小鼠。在所有组的肝、肾和血清样本中研究了主要抗氧化酶、抗氧化蛋白、功能标志物、脂质过氧化和蛋白质氧化的状态。顺铂处理组的抗氧化酶水平显著降低，且脂质和蛋白质氧化程度更高。核黄素处理组也观察到类似但程度较轻的模式。顺铂和核黄素联合处理组的所有参数均呈剂量依赖性趋势，趋于正常水平。因此，可以假设在所述治疗条件下，核黄素对顺铂诱导的肾毒性和肝毒性具有改善作用。
