Hassen Marwa T, Mohamed Hanaa K, Montaser Metwally M, El-Sharnouby Mohamed E, Awad Nabil, Ebiya Rasha A
Department of Zoology, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo 11757, Egypt.
Science and Technology Department, University College of Ranyah, Taif University, Ranyah 21975, Saudi Arabia.
Animals (Basel). 2021 Apr 16;11(4):1142. doi: 10.3390/ani11041142.
Cisplatin (Cis) a drug commonly used as a chemotherapeutic agent to treat various types of cancer, inducing testicular damage. The present study aimed to investigate the inhibitory potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) and beetroot extract (BRE) in albino rats after testicular toxicity induced by cisplatin. Thirty adult male albino rats were grouped into: the control group, Cis group receiving a single dose of 7 mg/kg i.p. (intraperitoneal) to induce testicular toxicity, Cis plus BM-MSCs injected Cis followed by 2 × 10 of BM-MSCs; Cis plus BRE group receiving Cis followed by 300 mg/kg body weight/day of BRE, and Cis plus BM-MSCs and BRE group. In the current study, Cis reduced sperm count, serum testosterone level, and testicular activity of alkaline phosphatase (AKP), besides a marked inhibition of succinate dehydrogenase (SDH) activity. In addition, it significantly increased malondialdehyde (MDA) and along with a marked decrease in testis reduced glutathione content and total antioxidant capacity (TAC). At the same time, Cis administration resulted in a marked elevation in interleukine-6 and the iNOS and caspase-3 genes; however, it decreased the expression of steroidogenic acute regulatory protein (StAR). Combined treatment with BM-MSCs and BRE resulted in great improvement of all previous parameters. These results were also confirmed by histopathological and immunohistochemical examination. In conclusion, both MSCs and BRE were found to have potent potentials to inhibit testicular damage induced by cisplatin.
顺铂（Cis）是一种常用的化疗药物，用于治疗各种类型的癌症，但会导致睾丸损伤。本研究旨在探讨骨髓间充质干细胞（BM-MSCs）和甜菜根提取物（BRE）对顺铂诱导的睾丸毒性后的白化大鼠的抑制潜力。将30只成年雄性白化大鼠分为：对照组；顺铂组，腹腔注射单次剂量7 mg/kg诱导睾丸毒性；顺铂加BM-MSCs组，注射顺铂后再注射2×10个BM-MSCs；顺铂加BRE组，注射顺铂后给予300 mg/kg体重/天的BRE；顺铂加BM-MSCs和BRE组。在本研究中，顺铂降低了精子数量、血清睾酮水平和睾丸碱性磷酸酶（AKP）活性，同时显著抑制了琥珀酸脱氢酶（SDH）活性。此外，它还显著增加了丙二醛（MDA），同时睾丸中还原型谷胱甘肽含量和总抗氧化能力（TAC）显著降低。同时，顺铂给药导致白细胞介素-6以及诱导型一氧化氮合酶（iNOS）和半胱天冬酶-3基因显著升高；然而，它降低了类固醇生成急性调节蛋白（StAR）的表达。BM-MSCs和BRE联合治疗使所有上述参数都有了很大改善。这些结果也通过组织病理学和免疫组织化学检查得到了证实。总之，发现骨髓间充质干细胞和甜菜根提取物都有强大的潜力来抑制顺铂诱导的睾丸损伤。
