Salman Maria, Naseem Imrana
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, U.P- India 202002.
Front Biosci (Elite Ed). 2015 Jan 1;7(2):242-54. doi: 10.2741/E731.
Cisplatin used in treatment of solid tumor induces oxidative stress which leads to hepatotoxicity and nephrotoxicity. New strategies are therefore needed to combat toxicity and optimize its therapeutic potential. Riboflavin (VitaminB2) under photoillumination works as an anti proliferative agent and induces apoptosis. These properties of riboflavin have been exploited to mitigate cisplatin induced toxicities. 9,10-dimethylbenz(a)anthracene /12-O-tetradecanoylphorbol-13-acetate were used to induce skin tumor in Swiss albino mice. The tumor induced mice were treated with cisplatin, riboflavin as well as their combination under photo illumination. In comparison to tumor control group the cisplatin and riboflavin treated groups showed a compromised level of antioxidant enzymes, functional markers and a higher degree of lipid peroxidation. However these parameters tended towards normal in the combination treated group. The results from histopathology indicate that apoptosis was favored mode of cell death and that necrosis was reduced in combination treated groups. Our findings indicate that combination of cisplatin with riboflavin under photo illumination synergizes its anti cancer activity towards cancer cells and attenuates the cisplatin induced toxicities.
用于实体瘤治疗的顺铂会诱导氧化应激，进而导致肝毒性和肾毒性。因此，需要新的策略来对抗毒性并优化其治疗潜力。核黄素（维生素B2）在光照下可作为抗增殖剂并诱导细胞凋亡。核黄素的这些特性已被用于减轻顺铂诱导的毒性。使用9,10-二甲基苯并(a)蒽/12-O-十四烷酰佛波醇-13-乙酸酯诱导瑞士白化小鼠皮肤肿瘤。对诱导出肿瘤的小鼠在光照下用顺铂、核黄素及其组合进行治疗。与肿瘤对照组相比，顺铂和核黄素治疗组的抗氧化酶、功能标志物水平受损，脂质过氧化程度更高。然而，这些参数在联合治疗组中趋于正常。组织病理学结果表明，细胞凋亡是细胞死亡的主要方式，联合治疗组的坏死减少。我们的研究结果表明，光照下顺铂与核黄素联合使用可增强其对癌细胞的抗癌活性，并减轻顺铂诱导的毒性。
