Kandeil Mohamed A, Gomaa Safaa B, Mahmoud Mohamed O
Department of Biochemistry, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt.
Department of Biochemistry, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt.
Heliyon. 2020 Aug 26;6(8):e04708. doi: 10.1016/j.heliyon.2020.e04708. eCollection 2020 Aug.
Cisplatin (CP) is a common antineoplastic agent widely used to treat a broad spectrum of cancers. However, its usage for cancer treatment was restricted due to various side effects such as neurotoxicity, nephrotoxicity, hepatotoxicity and ototoxicity. Neurotoxicity in patients who have undergone a complete course of chemotherapy is clinically evident. CP administration caused problems in rats with memory and learning.
The effect of combination of CP with either thymoquinone (TQ) or geraniol (Ger) on cell viability of human breast cancer cells (MCF-7) was detected by MTT assay. Forty male Wistar albino rats, healthy and adult, were divided into four groups: normal control, CP-treated group, CP + TQ-treated group and CP + Ger-treated group.
Our results demonstrated that prophylactic treatment with either TQ or Ger plus CP enhanced the anticancer effect of CP in MCF-7 cell line. In vivo study showed that CP-treated rats had higher depressives like behavior in open field and Morris water maze test while prophylactic treatment with either TQ or Ger and CP significantly enhanced the performance of depressive-like behavior. Also, histopathological evaluation of brain tissues proved the neurotoxic effect of CP and the possible protective activity of either TQ or Ger.
The findings of the present work revealed that TQ or Ger along with CP may enhance the antitumor effect of CP. Also, spontaneous administration of CP with either TQ or Ger as natural antioxidants may prevent CP-induced neurotoxicity in rats through diminishing the memory and learning impairment.
顺铂（CP）是一种常用的抗肿瘤药物，广泛用于治疗多种癌症。然而，由于其具有神经毒性、肾毒性、肝毒性和耳毒性等多种副作用，其在癌症治疗中的应用受到限制。接受完整化疗疗程的患者会出现明显的临床神经毒性。给大鼠注射顺铂会导致其出现记忆和学习问题。
采用MTT法检测顺铂与百里醌（TQ）或香叶醇（Ger）联合使用对人乳腺癌细胞（MCF-7）细胞活力的影响。将40只健康成年雄性Wistar白化大鼠分为四组：正常对照组、顺铂治疗组、顺铂+百里醌治疗组和顺铂+香叶醇治疗组。
我们的结果表明，百里醌或香叶醇联合顺铂进行预防性治疗可增强顺铂对MCF-7细胞系的抗癌作用。体内研究表明，顺铂治疗的大鼠在旷场试验和莫里斯水迷宫试验中表现出更多类似抑郁的行为，而百里醌或香叶醇与顺铂的预防性治疗显著改善了类似抑郁行为的表现。此外，脑组织的组织病理学评估证实了顺铂的神经毒性作用以及百里醌或香叶醇可能的保护活性。
本研究结果表明，百里醌或香叶醇与顺铂联合使用可能增强顺铂的抗肿瘤作用。此外，将顺铂与百里醌或香叶醇作为天然抗氧化剂自发给药，可能通过减轻记忆和学习障碍来预防顺铂诱导的大鼠神经毒性。
