Department of Nutrition and Health Science, Chang Jung Christian University, Tainan 711, Taiwan.
Fish Shellfish Immunol. 2010 Aug;29(2):258-63. doi: 10.1016/j.fsi.2010.04.021. Epub 2010 May 10.
Respiratory burst, lysozyme and phagocytic activities, and immunoglobulin levels in response to the pathogen Aeromonas hydrophila were examined in tilapia (Oreochromis mossanbicus, 10.65 +/- 2.5 g) injected individually with hot-water extract of Toona sinensis at 4 or 8 microg g(-1). Tilapia receiving the hot-water extract of T. sinensis at either dose had significantly increased respiratory burst, phagocytic activity and lysozyme activity towards A. hydrophila by 1 and 2 days post injection. No significant differences in total immunoglobulin levels were observed among the tilapia that received the two different doses of hot-water extract of T. sinensis at 4 and 8 microg g(-1). In another experiment, a Tilapia that had been injected with hot-water extract of T. sinensis was challenged with A. hydrophila at 5 x 10(7) colony-forming units (cfu) fish(-1). The survival of tilapia that received the hot-water extract of T. sinensis at 8 microg g(-1) was significantly higher than fish that received phosphate buffered saline and the control fish after 2 days, and at the termination of the experiment (7 days after the challenge). It was concluded that the hot-water extract of T. sinensis at 8 microg g(-1) or less had increased the immune response and resistance to A. hydrophila infection in tilapia.
研究了经热水提取的香椿（Toona sinensis）以 4 或 8μg g（-1）剂量单独注射入罗非鱼（Oreochromis mossanbicus，10.65±2.5g）后，鱼体针对病原菌嗜水气单胞菌（Aeromonas hydrophila）的呼吸爆发、溶菌酶和吞噬活性以及免疫球蛋白水平的变化。注射了任一种剂量的热水提取香椿的罗非鱼在注射后第 1 和第 2 天，其呼吸爆发、吞噬活性和溶菌酶活性对 A. hydrophila 的活性显著增强。在接受 4 和 8μg g（-1）两种不同剂量的热水提取香椿的罗非鱼之间，总免疫球蛋白水平没有显著差异。在另一个实验中，用热水提取的香椿溶液注射罗非鱼，然后用 5×10（7）cfu 鱼（-1）的嗜水气单胞菌对其进行攻毒。注射了 8μg g（-1）剂量的热水提取香椿溶液的罗非鱼的存活率在攻毒后第 2 天和实验结束（攻毒后 7 天）时显著高于注射了磷酸盐缓冲液和对照组的罗非鱼。结果表明，8μg g（-1）或更低剂量的热水提取香椿溶液可增强罗非鱼针对 A. hydrophila 感染的免疫反应和抵抗力。
