Osadchaia A I, Safronova L A, Avdeeva L V, Iliash V M
Mikrobiol Z. 2009 Sep-Oct;71(5):41-8.
Screening of aerobic sporulating bacteria of Bacillus genus from the collection of IMV ofNAS of Ukraine was performed to search for producers of cellulosolytic enzymes. Only 206 strains (58.4%) of 353 studied bacteria strains are capable to decompose carboxymethylcellulose, 158 strains (44.8%)--cellobiose. Under these conditions 91 strains (25% of all studied ones) synthesized actively both endoglucanase and cellobiase. It was shown that biosynthesis of enzymes of cellulose complex in bacilli was of inducible character, white the composition and activity of its components was a regulated process and was determined both by physiologic features of strains and by the conditions of their cultivation, composition of nutrient medium in particular. The strains of bacilli with high level of fermentation activity were selected as a result of screening.
为了寻找纤维素分解酶的产生菌，对乌克兰国家科学院微生物与病毒研究所保藏的芽孢杆菌属需氧产芽孢细菌进行了筛选。在353株被研究的细菌菌株中，只有206株（58.4%）能够分解羧甲基纤维素，158株（44.8%）能够分解纤维二糖。在这些条件下，91株（占所有被研究菌株的25%）同时积极合成内切葡聚糖酶和纤维二糖酶。结果表明，芽孢杆菌中纤维素复合酶的生物合成具有诱导特性，而其组分的组成和活性是一个受调控的过程，并且由菌株的生理特性以及它们的培养条件（特别是营养培养基的组成）所决定。通过筛选，选出了具有高发酵活性水平的芽孢杆菌菌株。
