Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130, USA.
J Biomed Opt. 2010 Mar-Apr;15(2):021303. doi: 10.1117/1.3333545.
With a refined ultrasound-array-based real-time photoacoustic microscopy (UA-PAM) system, we demonstrate the feasibility of noninvasive in vivo imaging of human pulsatile dynamics. The system, capable of real-time B-scan imaging at 50 Hz and high-speed 3-D imaging, is validated by imaging the subcutaneous microvasculature in rats and humans. After the validation, a human artery around the palm-wrist area is imaged, and its pulsatile dynamics, including the arterial pulsatile motion and changes in hemoglobin concentration, is monitored with 20-ms B-scan imaging temporal resolution. To our knowledge, this is the first demonstration of real-time photoacoustic imaging of human physiological dynamics. Our results show that UA-PAM can potentially enable many new possibilities for studying functional and physiological dynamics in both preclinical and clinical imaging settings.
利用一种基于超声阵列的实时光声显微镜（UA-PAM）系统，我们展示了无创性活体人搏动动力学成像的可行性。该系统能够以 50 Hz 的实时 B 扫描成像和高速 3D 成像进行验证，通过对大鼠和人体的皮下微血管成像进行验证。验证后，对掌腕区域周围的一条人体动脉进行成像，并以 20 毫秒的 B 扫描成像时间分辨率监测其搏动动力学，包括动脉搏动运动和血红蛋白浓度变化。据我们所知，这是首例实时光声成像人体生理动力学的演示。我们的研究结果表明，UA-PAM 有可能为临床前和临床成像环境中的功能和生理动力学研究带来许多新的可能性。
