Lin Li, Yao Junjie, Zhang Ruiying, Chen Chun-Cheng, Huang Chih-Hsien, Li Yang, Wang Lidai, Chapman William, Zou Jun, Wang Lihong V
Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA.
Department of Biomedical Engineering, Duke University, Durham, NC, USA.
J Biophotonics. 2017 Jun;10(6-7):792-798. doi: 10.1002/jbio.201600236. Epub 2016 Dec 23.
We applied high-speed photoacoustic microscopy (PAM) for both cortical microenvironment studies and dynamic brain studies, with micrometer-level optical resolution and a millisecond-level cross-sectional imaging speed over a millimeter-level field of view. We monitored blood flow redistribution in mini-stroke mouse models and cerebral autoregulation induced by a vasoactive agent. Our results collectively suggest that high-speed PAM is a promising tool for understanding dynamic neurophysiological phenomena, complementing conventional imaging modalities.
我们将高速光声显微镜（PAM）应用于皮质微环境研究和动态脑研究，其具有微米级光学分辨率以及在毫米级视野上的毫秒级横截面成像速度。我们监测了小型中风小鼠模型中的血流再分布以及血管活性药物诱导的脑自动调节。我们的结果共同表明，高速PAM是理解动态神经生理现象的一种有前景的工具，可补充传统成像方式。
