Center for Grain and Animal Health Research, USDA-ARS, Manhattan, KS 66502, USA.
J Invertebr Pathol. 2010 Sep;105(1):112-5. doi: 10.1016/j.jip.2010.05.005. Epub 2010 May 9.
A microsporidium that closely resembles Paranosema species at the level of the light microscope was isolated from the rusty grain beetle, Cryptolestes ferrugineus. It's identity as Nosema oryzaephili (originally described from Oryzaephilus surinamensis) was confirmed by comparison with a known isolate of N. oryzaephili based on spore size, small subunit rDNA sequence, and relative infectivity to O. surinamensis, Tribolium castaneum, and Ephestia kuehniella. Phylogenetic analysis of the small subunit rDNA indicates clearly that this species belongs in the genus Paranosema and thus the designation Paranosema oryzaephili (Burges, Canning and Hurst) is proposed. In spite of the abundance, economic importance, and world-wide distribution of C. ferrugineus, this is the first report of a microsporidial infection in this species. This is also the first report of P. oryzaephili in the new world.
从锈赤扁谷盗（Cryptolestes ferrugineus）中分离到一种与在光学显微镜下的近缘种相似的微孢子虫。通过与已知的 N.oryzaephili 分离株进行比较，包括孢子大小、小亚基 rDNA 序列和对 O.surinamensis、Tribolium castaneum 和 Ephestia kuehniella 的相对感染力，确认其为稻瘿蚊（最初描述自拟谷盗）。小亚基 rDNA 的系统发育分析清楚地表明，该物种属于 Paranosema 属，因此提出 Paranosema oryzaephili（Burges、Canning 和 Hurst）的命名。尽管锈赤扁谷盗数量多、经济重要且分布广泛，但这是首次报道该物种感染微孢子虫。这也是新世界中首次报道 P.oryzaephili。
