Kacem H, Bakhoum A J S, Neifar L, Miquel J
Laboratoire de Biodiversité et Ecosystèmes Aquatiques, Département des Sciences de la Vie, Faculté des Sciences de Sfax, Sfax, Tunisia.
Parasitol Int. 2010 Sep;59(3):358-66. doi: 10.1016/j.parint.2010.04.008. Epub 2010 May 10.
Spermiogenesis and the ultrastructural organization of the spermatozoon of the digenean Neoapocreadium chabaudi are described by means of transmission electron microscopy. Spermiogenesis follows the usual pattern found in the digeneans. It begins with the formation of a zone of differentiation bordered by cortical microtubules, characterized by the presence of an intercentriolar body composed of seven electron-dense plates situated between two striated rootlets and two centrioles. These centrioles give rise to two free flagella. Later, both flagella undergo a rotation of 90 degrees and fuse with the median cytoplasmic process. Spermiogenesis finishes when the ring of arched membranes constricts. The mature spermatozoon of N. chabaudi is characterized by the presence of 2 axonemes of different lengths presenting the 9+"1" trepaxonematan pattern, 2 bundles of parallel cortical microtubules, 2 mitochondria, a nucleus, and granules of glycogen. Nevertheless, several characters such as the morphology of sperm extremities and the presence of spinelike bodies allow us to distinguish N. chabaudi from other digenetic trematodes. The present paper provides the first ultrastructural results of a digenean belonging to the family Apocreadiidae that may be useful for the understanding of digenean relationships and phylogenetic studies.
利用透射电子显微镜描述了双殖吸虫查氏新隐殖吸虫精子发生过程及精子的超微结构组织。精子发生遵循双殖吸虫中常见的模式。它始于由皮质微管界定的分化区的形成，其特征是存在一个由七个电子致密板组成的中心粒间体，位于两个横纹状小根和两个中心粒之间。这些中心粒产生两条游离鞭毛。后来，两条鞭毛都旋转90度并与中间细胞质突起融合。当拱形膜环收缩时，精子发生完成。查氏新隐殖吸虫的成熟精子特征是存在两条不同长度的轴丝，呈现9+“1”三辐轴丝模式，两束平行的皮质微管，两个线粒体，一个细胞核和糖原颗粒。然而，一些特征，如精子末端的形态和刺状物体的存在，使我们能够将查氏新隐殖吸虫与其他双殖吸虫区分开来。本文提供了属于隐殖科的一种双殖吸虫的首个超微结构结果，这可能有助于理解双殖吸虫的关系和系统发育研究。
