Miyaishi S
Department of Legal Medicine, Okayama University Medical School, Japan.
Nihon Hoigaku Zasshi. 1991 Feb;45(1):6-25.
We have devised an enzyme immunoassay for the determination of human myoglobin. This assay provides superb reproducibility and a recovery rate without the influence of any anti-coagulants and hemolysis. The mean blood myoglobin level in healthy humans, determined by this assay, was 35.5 +/- 23.9 ng/ml and the urine myoglobin level was found to be distributed over a broad range under 373.5 ng/ml. In cases of trauma sustained by living bodies, the blood myoglobin level was found to increase in accordance to the severity of trauma. Further, the myoglobin level in the blood began its increase during the early stage of the injury, and greater increases were shown in the elderly. In autopsy specimens, the ratio of the cardiac blood myoglobin level to the cerebrospinal fluid myoglobin level had a negative correlation with the postmortem passage of time and, as in the case of living bodies, the myoglobin level in the blood was considered a useful indicator of the severity of damage. However, since myoglobin level was found to vary, depending on the sites from where the sample blood was taken, the evaluation of the data should be done carefully. Finally, the simultaneous determination of the creatine phosphokinase (CPK) activity in autopsied blood samples would be of help for estimating the postmortem passage of time and the progress of putrefaction.
我们设计了一种用于测定人肌红蛋白的酶免疫测定法。该测定法具有出色的重现性且回收率不受任何抗凝剂和溶血的影响。通过此测定法测定的健康人血液中肌红蛋白的平均水平为35.5±23.9 ng/ml，尿液中肌红蛋白水平在373.5 ng/ml以下分布范围较广。在活体遭受创伤的情况下，发现血液中肌红蛋白水平会根据创伤的严重程度而升高。此外，血液中的肌红蛋白水平在损伤早期就开始升高，老年人升高得更明显。在尸检标本中，心脏血液中肌红蛋白水平与脑脊液中肌红蛋白水平的比值与死后经过时间呈负相关，并且与活体情况一样，血液中的肌红蛋白水平被认为是损伤严重程度的有用指标。然而，由于发现肌红蛋白水平会因采集血样的部位不同而有所变化，因此对数据的评估应谨慎进行。最后，同时测定尸检血样中的肌酸磷酸激酶（CPK）活性将有助于估计死后经过时间和腐败进程。
