Miyaishi S, Moriya F, Yamamoto Y, Kitao T, Ishizu H
Department of Legal Medicine, Okayama University Medical School, Japan.
Nihon Hoigaku Zasshi. 1994 Dec;48(6):433-8.
A method for discriminating between postmortem and antemortem blood from bloodstains by detection of human myoglobin using a dot-ELISA was devised, and its applicability to forensic practice was investigated. This method exploits the high amount of myoglobin present in postmortem blood in comparison with that in antemortem blood. Our dot-ELISA was able to detect human myoglobin from bloodstains containing more than 10 micrograms/ml myoglobin, the level commonly observed in postmortem blood. Using this method, 10 stains of postmortem blood and 10 of antemortem blood were all identified correctly. A one-year-old stain made of postmortem blood and a stain of bloody fluid obtained from a severely putrefied body 4 months after death were identified as postmortem blood by this method. Two practical cases for which this method was applied are presented.
设计了一种通过斑点酶联免疫吸附测定法检测人肌红蛋白来区分血迹中死后血液和生前血液的方法，并研究了其在法医实践中的适用性。该方法利用了死后血液中肌红蛋白含量高于生前血液这一特点。我们的斑点酶联免疫吸附测定法能够从每毫升含有超过10微克肌红蛋白的血迹中检测到人肌红蛋白，这是死后血液中常见的水平。使用该方法，10份死后血液样本和10份生前血液样本均被正确鉴定。一份保存一年的死后血液样本以及一份在死后4个月从严重腐败尸体获取的血性液体样本，通过该方法被鉴定为死后血液。本文还介绍了应用该方法的两个实际案例。
