Department of Food Science, Cornell University, Ithaca, New York 14853-7201, USA.
J Agric Food Chem. 2010 Jun 9;58(11):6621-9. doi: 10.1021/jf9035832.
The measurement of antioxidant activity using biologically relevant assays is important to screen fruits, vegetables, natural products, and dietary supplements for potential health benefits. The cellular antioxidant activity (CAA) assay quantifies antioxidant activity using a cell culture model and was developed to meet the need for a more biologically representative method than the popular chemistry antioxidant capacity measures. The objective of the study was to determine the CAA, total phenolic contents, and oxygen radical absorbance capacity (ORAC) values of 27 vegetables commonly consumed in the United States. Beets, broccoli, and red pepper had the highest CAA values, whereas cucumber had the lowest. CAA values were significantly correlated to total phenolic content. Potatoes were found to be the largest contributors of vegetable phenolics and CAA to the American diet. Increased fruit and vegetable consumption is an effective strategy to increase antioxidant intake and decrease oxidative stress and may lead to reduced risk of developing chronic diseases, such as cancer and cardiovascular disease.
使用与生物学相关的测定方法来衡量抗氧化活性对于筛选水果、蔬菜、天然产物和膳食补充剂以获得潜在的健康益处非常重要。细胞抗氧化活性 (CAA) 测定法使用细胞培养模型来量化抗氧化活性，它的开发是为了满足对比流行的化学抗氧化能力测定法更具生物学代表性的方法的需求。本研究的目的是测定美国常见的 27 种蔬菜的 CAA、总酚含量和氧自由基吸收能力 (ORAC) 值。甜菜、西兰花和红辣椒的 CAA 值最高，而黄瓜的 CAA 值最低。CAA 值与总酚含量呈显著正相关。研究发现，土豆是美国人饮食中蔬菜酚类和 CAA 的最大贡献者。增加水果和蔬菜的摄入量是增加抗氧化剂摄入、减少氧化应激的有效策略，可能会降低患癌症和心血管疾病等慢性疾病的风险。
