Muehleisen Beda, Schaerer Leo, Dummer Reinhard, Burg Günter, Hofbauer Günther F L
Department of Dermatology, University Hospital Zürich, Zürich, Switzerland.
J Cutan Pathol. 2007 Jan;34(1):1-6. doi: 10.1111/j.1600-0560.2006.00576.x.
Lifetime risk for squamous cell carcinoma (SCC) of the skin is 1:30. Risk in organ-transplant recipients (OTR) is increased over 60-fold through long-term drug-induced immunosuppression. MAGE family-derived peptides are cancer/testis antigens recognized by specific CD8(+) T cells and employed for immunotherapy. We were interested in the frequency and distribution of MAGE-A4 in epithelial skin tumors of OTR and immunocompetent patients.
mAb 57B predominantly recognizing MAGE-A4 was used to stain 119 formalin-fixed, paraffin-embedded epithelial skin tumors (actinic keratosis, bowenoid actinic keratosis, Bowen's disease, and SCC; n = 17, 25, 61, 16, respectively) in immunocompetent patients (n = 84) and OTR (n = 35).
All four epithelial skin tumors showed comparable immunoreactivity ranging from (25-71%, p = 0.361). Scattered immunoexpression pattern was more frequent in OTR (p = 0.025). SCC showed polarized immunoreactivity basally (p = 0.002).
MAGE-A4 was expressed in a large part of epithelial skin tumors with predominantly scattered immunoexpression pattern in OTR. The difference in immunoexpression pattern for immune status was limited, suggesting important non-immunosuppressor-mediated mechanisms for increased skin carcinogenesis in OTR. mAb 57B may be a helpful tool for immunohistochemistry and micrographic surgery using formalin-fixed paraffin-embedded tissue.
皮肤鳞状细胞癌（SCC）的终生风险为1:30。器官移植受者（OTR）由于长期药物诱导的免疫抑制，其风险增加了60多倍。MAGE家族衍生肽是被特定CD8(+) T细胞识别并用于免疫治疗的癌胚抗原。我们对OTR和免疫功能正常患者的上皮性皮肤肿瘤中MAGE-A4的频率和分布感兴趣。
使用主要识别MAGE-A4的单克隆抗体57B对免疫功能正常患者（n = 84）和OTR（n = 35）的119例福尔马林固定、石蜡包埋的上皮性皮肤肿瘤（光化性角化病、鲍温样光化性角化病、鲍温病和SCC；分别为n = 17、25、61、16）进行染色。
所有四种上皮性皮肤肿瘤均显示出相当的免疫反应性，范围为（25 - 71%，p = 0.361）。散在免疫表达模式在OTR中更常见（p = 0.025）。SCC在基底显示出极化免疫反应性（p = 0.002）。
MAGE-A4在大部分上皮性皮肤肿瘤中表达，在OTR中主要为散在免疫表达模式。免疫状态下免疫表达模式的差异有限，提示OTR皮肤癌发生增加存在重要的非免疫抑制介导机制。单克隆抗体57B可能是使用福尔马林固定石蜡包埋组织进行免疫组织化学和显微手术的有用工具。
