Department of Dermatology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Pathol Res Pract. 2011 Jan 15;207(1):43-8. doi: 10.1016/j.prp.2010.10.007. Epub 2010 Dec 7.
C-C chemokine receptor (CCR)10 is a specific receptor for chemokine ligand (CCL)27, a selective chemoattractant for skin-associated memory T cells to cutaneous sites. In melanoma, CCR10 increases the ability of neoplastic cells to grow, invade tissues, disseminate to lymph nodes, and escape the host immune responses. In this study, we investigated the expression of CCR10 and its ligand CCL27 in squamous cell carcinoma (SCC). CCR10 and CCL27 were expressed in SCC, actinic keratosis (AK), Bowen's disease, and seborrheic keratosis (predominantly prickle cell type), but not in seborrheic keratosis (predominantly basal cell type) and basal cell carcinoma. Furthermore, CCR10 and CCL27 were overexpressed in SCC relative to Bowen's disease, an early stage of SCC. Consistently, a human SCC cell line, A253 cells, and HaCaT cells exhibited CCL27 production that was strongly induced by tumor necrosis factor-α and interleukin-1β. Finally, A253 cells expressed stronger intracellular CCR10 compared to HaCaT cells by flow cytometry. These results suggest that CCR10 and CCL27 overexpression in SCC is related to the progression of SCC and is useful for the diagnosis of SCC.
C-C 趋化因子受体 (CCR)10 是趋化因子配体 (CCL)27 的特异性受体，CCL27 是皮肤相关记忆 T 细胞向皮肤部位趋化的选择性趋化因子。在黑色素瘤中，CCR10 增加了肿瘤细胞生长、侵袭组织、向淋巴结扩散以及逃避宿主免疫反应的能力。在这项研究中，我们研究了 CCR10 及其配体 CCL27 在鳞状细胞癌 (SCC) 中的表达。CCR10 和 CCL27 在 SCC、光化性角化病 (AK)、鲍文病和脂溢性角化病（主要为棘细胞型）中表达，但在脂溢性角化病（主要为基底细胞型）和基底细胞癌中不表达。此外，与鲍文病（SCC 的早期阶段）相比，CCR10 和 CCL27 在 SCC 中过度表达。一致地，人 SCC 细胞系 A253 细胞和 HaCaT 细胞表现出 CCL27 的产生，其强烈受到肿瘤坏死因子-α和白细胞介素-1β的诱导。最后，通过流式细胞术，A253 细胞表达比 HaCaT 细胞更强的细胞内 CCR10。这些结果表明，SCC 中 CCR10 和 CCL27 的过度表达与 SCC 的进展有关，对 SCC 的诊断有用。
