Food Science and Technology Department, Islamic Azad University-Quchan Branch, Khorasan Province, Iran.
Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2130-2. doi: 10.1016/j.fct.2010.05.015. Epub 2010 May 22.
The aim of this study was to evaluate aflatoxin M(1) (AFM(1)) contamination and antibiotic presence in milk samples in Khorasan province in Iran. During 4 months (March to June 2008), one hundred ninety-six milk samples were collected from seven dairies. The occurrence and concentration range of AFM(1) in the samples were investigated by competitive enzyme-linked immunoabsorbent assay (ELISA) method. Antibiotic presence was determined using Copan test, a broad-spectrum test capable of detecting beta-lactams, tetracyclines, sulfonamides, aminoglycosides and macrolydes. AFM(1) was found in 100% of the examined milk samples by average concentration of 77.92 ng/kg. The concentration of AFM(1) in all of the samples were lower than Iranian national standard and FDA limit (500 ng/L), but 80.6% of the samples had AFM(1) greater than the maximum tolerance limit (50 ng/L) accepted by European Union and Codex Alimentarius Commission. Statistical evaluation showed no significant difference between the mean concentrations of AFM(1) of milk samples taken from different factories (P>0.05). Copan milk test was positive for 40.8% of the samples.
本研究旨在评估伊朗霍拉桑省牛奶样本中黄曲霉毒素 M1 (AFM1) 的污染和抗生素的存在情况。在 2008 年 3 月至 6 月的 4 个月期间，从 7 家奶牛场采集了 196 个牛奶样本。采用竞争酶联免疫吸附试验 (ELISA) 方法检测样本中 AFM1 的存在和浓度范围。Copan 测试用于检测抗生素的存在，这是一种能够检测β-内酰胺类、四环素类、磺胺类、氨基糖苷类和大环内酯类的广谱测试。通过平均浓度为 77.92ng/kg，发现 100%的检测牛奶样本中存在 AFM1。所有样本中 AFM1 的浓度均低于伊朗国家标准和 FDA 限量（500ng/L），但 80.6%的样本中 AFM1 的浓度高于欧盟和食品法典委员会接受的最大耐受限量（50ng/L）。统计评估显示，不同工厂采集的牛奶样本中 AFM1 的平均浓度无显著差异（P>0.05）。Copan 牛奶测试对 40.8%的样本呈阳性。
