Oliveira C A, Rosmaninho J, Rosim R
Food Addit Contam. 2006 Feb;23(2):196-201. doi: 10.1080/02652030500398379.
Aflatoxin M(1) (AFM(1)) and cyclopiazonic acid (CPA) were determined in 48 samples of grade A-C and ultra-high-temperature (UHT)-treated fluid commercial milk from São Paulo, Brazil, from July to December 2004. AFM(1) and CPA determinations were carried out by HPLC. Results showed 37 (77.1%) positive samples for AFM(1) at levels of 0.011-0.251 microg l(-1) milk, which were below the tolerance limit for AFM(1) in milk as adopted by Brazilian regulations. Mean levels of AFM(1) in grades A-C and UHT milk were 0.060+/-0.080, 0.056+/-0.063, 0.064+/-0.041 and 0.075+/-0.048microg l(-1), respectively. CPA was detected in two samples (4.2%) of grade A milk at levels of 6.4 and 9.7 microg l(-1). This is the first report of the occurrence of CPA in milk in Brazil. It was concluded that the incidence of AFM(1) in milk traded in São Paulo is low, but the simultaneous occurrence of CPA, even at low levels, warrants concern about AFM(1) in milk and milk products.
2004年7月至12月，对巴西圣保罗市48份A - C级和超高温（UHT）处理的市售液态奶样品进行了黄曲霉毒素M1（AFM1）和环匹阿尼酸（CPA）检测。采用高效液相色谱法（HPLC）测定AFM1和CPA。结果显示，37份（77.1%）样品的AFM1呈阳性，牛奶中AFM1含量为0.011 - 0.251微克/升，低于巴西法规规定的牛奶中AFM1的耐受限量。A - C级牛奶和UHT奶中AFM1的平均含量分别为0.060±0.080、0.056±0.063、0.064±0.041和0.075±0.048微克/升。在两份（4.2%）A级牛奶样品中检测到CPA，含量分别为6.4和9.7微克/升。这是巴西首次报道牛奶中出现CPA。得出的结论是，圣保罗市交易的牛奶中AFM1的发生率较低，但即使CPA含量较低，其同时出现也值得关注牛奶及奶制品中的AFM1。
