College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
Magn Reson Chem. 2010 Jun;48(6):490-5. doi: 10.1002/mrc.2602.
Five new beta-carboline alkaloids, 6,12-dimethoxy-3-(2-hydroxylethyl)-beta-carboline (1), 3,10-dihydroxy-beta-carboline (2), 6,12-dimethoxy-3-(1-hydroxylethyl)-beta-carboline (3), 6,12-dimethoxy-3-(1,2-dihydroxylethyl)-beta-carboline (4), and 6-methoxy-3-(2-hydroxyl-1-ethoxylethyl)-beta-carboline (5), and two new natural products, 6-methoxy-12-hydroxy-3-methoxycarbonyl-beta-carboline (6) and 3-hydroxy-beta-carboline (7) were isolated from the stems of Picrasma quassioides along with 16 known beta-carboline alkaloids (8-23). The structures of new compounds were determined by extensive spectroscopic analyses, and the 1D and 2D NMR data of compounds 6, 7 and 10 were reported for the first time. The bioassays showed that only compounds 14 and 16 could enhance the differentiation of 3T3-L1 preadiocytes accompanied by secretion of adiponectin proteins among these 23 compounds.
从密蒙花的茎中分离得到了 23 种β-咔啉生物碱，其中包括 5 种新的β-咔啉生物碱：6,12-二甲氧基-3-(2-羟乙基)-β-咔啉（1）、3,10-二羟基-β-咔啉（2）、6,12-二甲氧基-3-(1-羟乙基)-β-咔啉（3）、6,12-二甲氧基-3-(1,2-二羟乙基)-β-咔啉（4）和 6-甲氧基-3-(2-羟乙基-1-乙氧基乙基)-β-咔啉（5），以及 2 种新的天然产物：6-甲氧基-12-羟基-3-甲氧基羰基-β-咔啉（6）和 3-羟基-β-咔啉（7）。新化合物的结构通过广泛的光谱分析确定，化合物 6、7 和 10 的 1D 和 2D NMR 数据也是首次报道。生物测定结果表明，在这 23 种化合物中，只有化合物 14 和 16 能够促进 3T3-L1 前体脂肪细胞的分化，并伴随着脂联素蛋白的分泌。
