Qiu Yi, Guo Qi, Ran Yan-Qin, Lan Wen-Jian, Lam Chi-Keung, Feng Gong-Kan, Deng Rong, Zhu Xiao-Feng, Li Hou-Jin, Chen Liu-Ping
School of Chemistry, Sun Yat-sen University Guangzhou 510275 China
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006 China.
RSC Adv. 2020 Jan 27;10(8):4243-4250. doi: 10.1039/c9ra10306f. eCollection 2020 Jan 24.
Eight different culture media were used to culture shellfish associated fungus sp. XBB-4. In a glucose-peptone-yeast (GPY) culture medium supplied with amino acids, this fungus can produce chemodiversity metabolites. Four new alkaloids including three β-carboline alkaloids, aspercarbolines A-C (1-3) and one piperazinedione, asperdione A (13) along with nine known compounds were isolated. The structures were elucidated mainly based on the NMR, MS, ECD and X-ray single-crystal diffraction data. The possible biosynthetic pathways of aspercarbolines A-C (1-3) were proposed. All compounds (1-13) were evaluated for their cytotoxicity against six cancer cell lines, including human nasopharyngeal carcinoma cell lines CNE1, CNE2, HONE1 and SUNE1, and human hepatocellular carcinoma cell lines hepG2 and QGY7701.
使用八种不同的培养基培养与贝类相关的真菌XBB-4菌株。在添加了氨基酸的葡萄糖-蛋白胨-酵母（GPY）培养基中，这种真菌能够产生具有化学多样性的代谢产物。从中分离出了四种新生物碱，包括三种β-咔啉生物碱，aspercarbolines A-C（1-3）和一种哌嗪二酮，asperdione A（13）以及九种已知化合物。主要基于核磁共振（NMR）、质谱（MS）、电子圆二色光谱（ECD）和X射线单晶衍射数据阐明了这些化合物的结构。提出了aspercarbolines A-C（1-3）可能的生物合成途径。评估了所有化合物（1-13）对六种癌细胞系的细胞毒性，包括人鼻咽癌细胞系CNE1、CNE2、HONE1和SUNE1，以及人肝癌细胞系hepG2和QGY7701。
