Bailey J E
California Institute of Technology, Pasadena 91125.
Science. 1991 Jun 21;252(5013):1668-75. doi: 10.1126/science.2047876.
Application of recombinant DNA methods to restructure metabolic networks can improve production of metabolite and protein products by altering pathway distributions and rates. Recruitment of heterologous proteins enables extension of existing pathways to obtain new chemical products, alter posttranslational protein processing, and degrade recalcitrant wastes. Although some of the experimental and mathematical tools required for rational metabolic engineering are available, complex cellular responses to genetic perturbations can complicate predictive design.
应用重组DNA方法来重构代谢网络，可以通过改变途径分布和速率来提高代谢物和蛋白质产物的产量。引入异源蛋白质能够扩展现有途径以获得新的化学产物、改变蛋白质翻译后加工过程以及降解难降解废物。尽管合理的代谢工程所需的一些实验和数学工具已经具备，但细胞对基因扰动的复杂反应可能会使预测性设计变得复杂。
