Ribeiro Deise H B, Ferreira Fabiane L, da Silva Valéria N, Aquino Simone, Corrêa Benedito
Centro de P&D de Proteção Ambiental, Instituto Biológico, São Paulo, SP, Brazil.
Int J Mol Sci. 2010 Apr 26;11(4):1944-55. doi: 10.3390/ijms11041944.
The present study evaluated the effect of aflatoxin B(1) (AFB(1)) and fumonisin B(1) (FB(1)) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB(1) and FB(1) used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner.
本研究评估了黄曲霉毒素B(1)（AFB(1)）和伏马菌素B(1)（FB(1)）单独或联合使用对大鼠原代肝细胞培养物的影响。通过碘化丙啶嵌入后用流式细胞术评估细胞活力。通过琼脂糖凝胶电泳以及吖啶橙和溴化乙锭染色评估DNA片段化和细胞凋亡。在所使用的AFB(1)和FB(1)浓度下，这些毒素并未降低细胞活力，但确实以浓度和时间依赖性方式诱导了细胞凋亡。
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