Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba 5000, Argentina.
Toxicology. 2010 Jan 31;268(1-2):104-10. doi: 10.1016/j.tox.2009.12.007. Epub 2009 Dec 14.
Some evidence suggests that fumonisin B(1) (FB(1)), a worldwide toxic contaminant of grains produced by Fusarium verticillioides, exhibits an oxidative stress mediated genotoxicity. We studied the DNA damage (by the alkaline comet and the micronucleus tests) and biomarkers of cellular oxidative stress (malondialdehyde, MDA; catalase, CAT; and superoxide dismutase, SOD) in spleen mononuclear cells of male Wistar rats subchronically (90 days) fed on a control experimental diet (CED) or poisoned with experimental diets contaminated with a culture material containing 100 ppm of FB(1) (FED), with 40 ppb of aflatoxin B(1) (a common toxic co-contaminant in cereals, AFB(1)ED), and with a mixture of both toxins (MED). The DNA damage was found in 13.7%, 81.7%, 98.0% and 99.3% (comet assay) and in 2.8%, 7.0%, 10.8% and 8.8% (micronucleus technique) in groups CED, FED, AFB(1)ED and MED, respectively. The MDA levels as well as the CAT and SOD activities were increased in all the poisoned animals. A similar behavior was observed in cells exposed in vitro to the toxins. These data support the hypothesis of an oxidative stress mediated genotoxicity induced by FB(1). Furthermore, the extent of DNA damage assessed by the comet assay suggests a possible protective effect of the fumonisins-AFB(1) mixtures in vitro against the genotoxicity induced individually by the toxins.
一些证据表明，由串珠镰刀菌产生的一种世界性谷物毒素——伏马菌素 B(1)（FB(1)），表现出氧化应激介导的遗传毒性。我们研究了雄性 Wistar 大鼠脾单核细胞中的 DNA 损伤（通过碱性彗星和微核试验）和细胞氧化应激生物标志物（丙二醛，MDA；过氧化氢酶，CAT；和超氧化物歧化酶，SOD），这些大鼠在喂食对照实验饮食（CED）或喂食含有 100ppm FB(1)（FED）、40ppb 黄曲霉毒素 B(1)（谷物中常见的毒性共污染物，AFB(1)ED）和两种毒素混合物（MED）的实验饮食的亚慢性（90 天）条件下。在 comet 试验中，分别在 CED、FED、AFB(1)ED 和 MED 组中发现了 13.7%、81.7%、98.0%和 99.3%的 DNA 损伤，在微核技术中发现了 2.8%、7.0%、10.8%和 8.8%的 DNA 损伤。所有中毒动物的 MDA 水平以及 CAT 和 SOD 活性均升高。在体外暴露于毒素的细胞中观察到类似的行为。这些数据支持了由 FB(1)引起的氧化应激介导遗传毒性的假设。此外，通过彗星试验评估的 DNA 损伤程度表明，在体外，伏马菌素-黄曲霉毒素混合物对毒素单独诱导的遗传毒性可能具有保护作用。
