细菌群集的动力学。
Dynamics of bacterial swarming.
机构信息
Rowland Institute at Harvard University, Cambridge, Massachusetts, USA.
出版信息
Biophys J. 2010 May 19;98(10):2082-90. doi: 10.1016/j.bpj.2010.01.053.
When vegetative bacteria that can swim are grown in a rich medium on an agar surface, they become multinucleate, elongate, synthesize large numbers of flagella, produce wetting agents, and move across the surface in coordinated packs: they swarm. We examined the motion of swarming Escherichia coli, comparing the motion of individual cells to their motion during swimming. Swarming cells' speeds are comparable to bulk swimming speeds, but very broadly distributed. Their speeds and orientations are correlated over a short distance (several cell lengths), but this correlation is not isotropic. We observe the swirling that is conspicuous in many swarming systems, probably due to increasingly long-lived correlations among cells that associate into groups. The normal run-tumble behavior seen in swimming chemotaxis is largely suppressed, instead, cells are continually reoriented by random jostling by their neighbors, randomizing their directions in a few tenths of a second. At the edge of the swarm, cells often pause, then swim back toward the center of the swarm or along its edge. Local alignment among cells, a necessary condition of many flocking theories, is accomplished by cell body collisions and/or short-range hydrodynamic interactions.
当能够游动的营养细菌在琼脂表面的丰富培养基中生长时,它们会变成多核、伸长、合成大量鞭毛、产生润湿剂,并在协调的群体中在表面上移动:它们会群集。我们研究了群集大肠杆菌的运动,将单个细胞的运动与它们在游泳时的运动进行了比较。群集细胞的速度与整体游泳速度相当,但分布非常广泛。它们的速度和方向在短距离(几个细胞长度)内相关,但这种相关性不是各向同性的。我们观察到了在许多群集系统中很明显的旋转,可能是由于细胞之间的关联越来越长寿命的相关性,这些细胞会聚集在一起。在游泳趋化性中看到的正常的翻滚行为在很大程度上被抑制了,相反,细胞会被它们的邻居随机地推挤,在几十分之一秒内随机改变它们的方向。在群集的边缘,细胞经常会暂停,然后游回群集的中心或沿着其边缘。细胞之间的局部对齐,是许多群集理论的必要条件,通过细胞体碰撞和/或短程水动力相互作用来实现。