College of Pharmacy, Chungbuk National University, Cheongju, Republic of Korea.
Bioorg Med Chem Lett. 2010 Jun 15;20(12):3785-7. doi: 10.1016/j.bmcl.2010.04.054. Epub 2010 Apr 24.
Bioactivity-guided isolation of the methanol extract of the stems of Dendrobium nobile yielded a new phenanthrene together with nine known phenanthrenes and three known bibenzyls. Their structures were elucidated by analysis of the spectroscopic data including 2D-NMR. All of the isolates were evaluated for their potential to inhibit the LPS-induced production of nitric oxide in murine macrophage RAW 264.7 cells. Compounds 1-4, 7-13 inhibited nitric oxide production with the IC(50) values ranging from 9.6 microM to 35.7 microM.
采用生物活性导向方法，从铁皮石斛茎的甲醇提取物中分离得到一个新的菲类化合物，以及 9 个已知的菲类化合物和 3 个已知的二苄基类化合物。通过分析包括 2D-NMR 在内的光谱数据，阐明了它们的结构。所有分离得到的化合物都被评估了其在 LPS 诱导的 RAW 264.7 巨噬细胞中抑制一氧化氮生成的潜力。化合物 1-4、7-13 对一氧化氮的生成具有抑制作用，IC50 值范围为 9.6 microM 至 35.7 microM。
