Badalamenti Natale, Russi Sabino, Bruno Maurizio, Maresca Viviana, Vaglica Alessandro, Ilardi Vincenzo, Zanfardino Anna, Di Napoli Michela, Varcamonti Mario, Cianciullo Piergiorgio, Calice Giovanni, Laurino Simona, Falco Geppino, Basile Adriana
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Università degli Studi di Palermo, Viale delle Scienze, ed. 17, 90128 Palermo, Italy.
IRCCS CROB-Referral Cancer Center of Basilicata, 85028 Rionero in Vulture, Italy.
Plants (Basel). 2021 Dec 15;10(12):2776. doi: 10.3390/plants10122776.
The peculiar aspect that emerges from the study of Orchidaceae is the presence of various molecules, which are particularly interesting for pharmaceutical chemistry due to their wide range of biological resources. The aim of our study was to investigate the properties of two dihydrophenanthrenes, isolated, for the first time, from (Loisel.) P. Delforge (Orchidaceae) bulbs and roots. Chemical and spectroscopic study of the bulbs and roots of (Loisel.) P. Delforge resulted in the isolation of two known dihydrophenanthrenes-loroglossol and hircinol-never isolated from this plant species. The structures were evaluated based on H-NMR, C-NMR, and two-dimensional spectra, and by comparison with the literature. These two molecules have been tested for their possible antioxidant, antimicrobial, antiproliferative, and proapoptotic activities. In particular, it has been shown that these molecules cause an increase in the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) in polymorphonuclear leukocytes (PMN); show antimicrobial activity against and , and have anti-proliferative effects on gastric cancer cell lines, inducing apoptosis effects. Therefore, these two molecules could be considered promising candidates for pharmaceutical and nutraceutical preparations.
从兰科植物的研究中出现的一个特殊方面是存在各种分子，由于它们广泛的生物来源，这些分子对药物化学特别有意义。我们研究的目的是研究首次从（洛塞尔）P.德尔福热（兰科）球茎和根中分离出的两种二氢菲的性质。对（洛塞尔）P.德尔福热的球茎和根进行化学和光谱研究，结果分离出两种已知的二氢菲——洛戈洛索和希尔西诺——从未从该植物物种中分离出来过。通过¹H-NMR、¹³C-NMR和二维光谱，并与文献进行比较对其结构进行了评估。对这两种分子的抗氧化、抗菌、抗增殖和促凋亡活性进行了测试。特别是，已表明这些分子会导致多形核白细胞（PMN）中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽S-转移酶（GST）的活性增加；对金黄色葡萄球菌和大肠杆菌显示出抗菌活性，并对胃癌细胞系具有抗增殖作用，诱导凋亡效应。因此，这两种分子可被认为是药物和营养制剂的有前途的候选物。
