冈比亚按蚊浓核病毒作为一种“进化-proof”生物农药的潜力。
Potential for the Anopheles gambiae densonucleosis virus to act as an "evolution-proof" biopesticide.
机构信息
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
出版信息
J Virol. 2010 Aug;84(15):7726-9. doi: 10.1128/JVI.00631-10. Epub 2010 May 19.
Abstract
"Evolution-proof" or "late-life-acting" insecticides (LLAIs) preferentially kill older adult mosquitoes and are of extreme interest to control vector-borne diseases such as malaria. We used quantitative PCR to assess whether the Anopheles gambiae densonucleosis virus (AgDNV) had potential as an LLAI. After infection, AgDNV titers increased modestly during larval development but replicated slower than the host cells, resulting in a significant decrease in the normalized virus titer during larval and pupal development. Normalized virus titers dramatically increased after adult emergence, peaking in 7- to 10-day-old adults. Unlike other DNVs, AgDNV does not significantly replicate in preadult mosquitoes but rather preferentially replicates in older adults. The natural dynamics of AgDNV make it ideal for expression of insect-specific toxin genes as a biological LLAI.
摘要
"抗进化"或"晚龄期作用"（LLAIs）杀虫剂优先杀死老龄成蚊，对于控制疟疾等蚊媒疾病具有极大的兴趣。我们使用定量 PCR 来评估是否携带 Gambiae 浓核病毒（AgDNV）有作为 LLAI 的潜力。感染后，AgDNV 的滴度在幼虫发育过程中略有增加，但比宿主细胞复制得更慢，导致幼虫和蛹发育过程中归一化病毒滴度显著下降。在成虫出现后，归一化病毒滴度急剧增加，在 7-10 天龄的成虫中达到峰值。与其他浓核病毒不同，AgDNV 不会在幼蚊中大量复制，而是优先在老龄成蚊中复制。AgDNV 的自然动态使其成为表达昆虫特异性毒素基因的理想生物 LLAI。
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