使用芽管试验对临床酵母分离株进行杜氏念珠菌筛查及韩国杜氏念珠菌的流行率。
Candida dubliniensis screening using the germ tube test in clinical yeast isolates and prevalence of C. dubliniensis in Korea.
机构信息
Department of Urology, Chung-Ang University College of Medicine, Seoul, Korea.
出版信息
J Clin Lab Anal. 2010;24(3):145-8. doi: 10.1002/jcla.20373.
The aim of this study was to screen for C. dubliniensis using the germ tube test with human pooled serum (HPS) in clinical isolates and investigate the prevalence of C. dubliniensis in Korea. Among 1,854 yeast strains isolated, 1,404 strains of C. albicans (on the basis of positive results of the germ tube test) and 192 germ tube-negative yeast strains were examined. All 1,596 clinical isolates were examined using the germ tube test with HPS, the differential temperature, and NaCl tolerance test. Only 81 isolates that did not grow at 45 degrees C nor on Sabouraud 6.5% NaCl broth were selected and tested using the VITEK 2 ID-YST system and the multiplex-PCR assay for the study. The two strains, C. dubliniensis ATCC MYA-646 and KCTC 17427 failed to produce germ tubes in HPS but produced them in fresh rabbit serum (FRS) and fetal bovine serum (FBS). No C. dubliniensis was found in this study population. The results of this study suggest that the germ tube test with HPS in combination with FRS or FBS can be used for discriminating between C. albicans and C. dubliniensis strains and that the prevalence of C. dubliniensis appears to be extremely low in Korea.
本研究旨在使用人混合血清（HPS）的芽管试验筛选杜氏利什曼原虫，并调查韩国杜氏利什曼原虫的流行情况。在分离出的 1854 株酵母菌株中，对 1404 株白色念珠菌（基于芽管试验阳性结果）和 192 株芽管阴性酵母菌株进行了检查。使用 HPS 的芽管试验、差温试验和 NaCl 耐受试验对 1596 株全部临床分离株进行了检查。仅选择未在 45°C 下生长且不在 Sabouraud 6.5%NaCl 肉汤中生长的 81 株分离株，并使用 VITEK 2 ID-YST 系统和多重 PCR 分析进行检测。两种菌株，杜氏利什曼原虫 ATCC MYA-646 和 KCTC 17427，在 HPS 中未能产生芽管，但在新鲜兔血清（FRS）和胎牛血清（FBS）中产生了芽管。在本研究人群中未发现杜氏利什曼原虫。本研究结果表明，HPS 联合 FRS 或 FBS 的芽管试验可用于区分白色念珠菌和杜氏利什曼原虫株，并且韩国的杜氏利什曼原虫的流行率似乎极低。
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