Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA.
J Clin Lab Anal. 2010;24(3):160-2. doi: 10.1002/jcla.20382.
Despite brucellosis having a low incidence rate in developed nations, it still remains the leading zoonotic disease in the world. Culturing of Brucella spp. provides good specificity but in cases where the fever is intermittent, sensitivity is problematic. This has led to the development of serological methods of detection. Brucella agglutination methods have been considered the serological gold-standard since their inception, although commercial Brucella IgG and IgM enzyme-linked immunosorbent assays are available to potentially aid in the diagnosis of the disease. In our study, anti-Brucella IgG and IgM assays were compared with agglutination. Individually the IgG assay tested had an accuracy of 56% and the IgM assay had an accuracy of 77%. These poor accuracies reinforce Centers for Disease Control's conclusion that nonagglutination tests should not be used to confirm brucellosis.
尽管布鲁氏菌病在发达国家的发病率较低，但它仍是世界上主要的动物源性传染病。布鲁氏菌属的培养提供了良好的特异性，但在发热间歇性的情况下，敏感性则存在问题。这导致了血清学检测方法的发展。自从布鲁氏菌凝集方法问世以来，它们一直被认为是血清学的金标准，尽管现在已有商业化的布鲁氏菌 IgG 和 IgM 酶联免疫吸附试验，可能有助于疾病的诊断。在我们的研究中，将抗布鲁氏菌 IgG 和 IgM 检测与凝集法进行了比较。单独检测 IgG 时的准确性为 56%，检测 IgM 时的准确性为 77%。这些较低的准确性证实了疾病控制中心的结论，即不应使用非凝集试验来确认布鲁氏菌病。
