Global Disease Detection and Response Program (GDDRP), US Naval Medical Research Unit No. 3 (NAMRU-3), PSC 452, Box 5000, FPO AE 09835-0007, Cairo, Egypt.
National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333, USA.
J Med Microbiol. 2011 Dec;60(Pt 12):1767-1773. doi: 10.1099/jmm.0.033381-0. Epub 2011 Aug 11.
Brucellosis is a worldwide zoonotic disease that often requires serology for diagnosis. The serum agglutination test is the gold standard assay, but ELISAs are used by many laboratories. Many commercial ELISAs are available, but few studies have compared their performance. This study compared the ability of four commercially available ELISA kits (from Bio-Quant, Immuno-Biological Laboratories - America, Vircell and Euroimmun) to diagnose brucellosis in patients from Egypt and the USA. The sensitivities for all kits tested, except the Vircell kit, were >90%, whilst the specificities were variable, with the Bio-Quant assay having a specificity of <40%. Detection of IgG antibody was more sensitive than detection of IgM antibody for diagnosing brucellosis cases, but the specificity was comparable. Overall, there was good agreement between all of the kits except for the Bio-Quant kit. None of the diagnostic assays was 100% reliable for diagnosing brucellosis; therefore, serology results need to be considered in tandem with patient history, clinical signs and other test results.
布鲁氏菌病是一种全球性的人畜共患病，通常需要血清学诊断。血清凝集试验是金标准检测方法，但许多实验室都使用 ELISA。有许多商业 ELISA 可用，但很少有研究比较它们的性能。本研究比较了来自 Bio-Quant、Immuno-Biological Laboratories - America、Vircell 和 Euroimmun 的四种市售 ELISA 试剂盒在诊断来自埃及和美国的患者布鲁氏菌病方面的能力。除了 Vircell 试剂盒外，所有测试试剂盒的敏感性均>90%，而特异性则各不相同，Bio-Quant 试剂盒的特异性<40%。与检测 IgM 抗体相比，检测 IgG 抗体对诊断布鲁氏菌病更敏感，但特异性相当。总体而言，除了 Bio-Quant 试剂盒外，所有试剂盒之间的一致性都很好。没有一种诊断检测方法对诊断布鲁氏菌病的可靠性达到 100%；因此，血清学结果需要与患者病史、临床症状和其他检测结果一起考虑。
