Operative Unit of Occupational Medicine of the University G. d'Annunzio of Chieti-Pescara, Chieti, Pescara, Italy.
J Biol Regul Homeost Agents. 2010 Apr-Jun;24(2):207-14.
The object of this study is to determine the cytokine release from PBMCs exposed to Pd model nanoparticles emitted from catalytic converters. PBMCs of 8 healthy non-atopic women were incubated in the presence of Pd nanoparticles (5-10 nm) or salt (potassium hexa-chloropalladate) 10-5 and 10-6 M. Release of cytokines in supernatant of PBMCs was then determined. In cultures without LPS, IL-10 and IL-17 release from PBMCs was inhibited by Pd salt, while Pd nanoparticles inhibited TNF-alpha and IL-17 release. In LPS-stimulated cultures, release of IFN-gamma, TNF-alpha, IL-10 and IL-17 was inhibited by Pd salt, whereas IFN-gamma release was enhanced and TNF-alpha and IL-17 release was inhibited by Pd nanoparticles. In conclusion, Pd salt inhibits cytokine release, whereas Pd nanoparticles exert modulatory effects enhancing the release of IFN-gamma, a Th1 cytokine typical of delayed allergic reactions. This result is interesting considering the increase of allergic contact dermatitis to Pd in people exposed to Pd nanoparticles in urban environments.
本研究的目的是确定 PBMC 在暴露于来自催化转化器的 Pd 模型纳米颗粒后细胞因子的释放。将 8 名健康非过敏女性的 PBMC 在存在 Pd 纳米颗粒（5-10nm）或盐（六氯钯酸钾）10-5 和 10-6M 的情况下孵育。然后测定 PBMC 上清液中细胞因子的释放。在没有 LPS 的培养物中，Pd 盐抑制 PBMC 释放 IL-10 和 IL-17，而 Pd 纳米颗粒抑制 TNF-α和 IL-17 的释放。在 LPS 刺激的培养物中，Pd 盐抑制 IFN-γ、TNF-α、IL-10 和 IL-17 的释放，而 Pd 纳米颗粒增强 IFN-γ的释放，并抑制 TNF-α和 IL-17 的释放。总之，Pd 盐抑制细胞因子的释放，而 Pd 纳米颗粒发挥调节作用，增强 Th1 细胞因子 IFN-γ的释放，这是迟发型过敏反应的典型特征。考虑到在城市环境中接触 Pd 纳米颗粒的人群中对 Pd 的过敏性接触性皮炎增加，这一结果很有趣。
