Sathaye Sadhana, Bagul Yogita, Gupta Sanjay, Kaur Harpreet, Redkar Roopali
Department of Pharmaceutical Science and Technology, Institute of Chemical Technology, University of Mumbai, N.P. Marg, Matunga, Mumbai 400019, Maharashtra, India.
Exp Toxicol Pathol. 2011 Sep;63(6):587-91. doi: 10.1016/j.etp.2010.04.012. Epub 2010 May 20.
Medicinal plants constitute a principal health care resource corroborating their gradual acceptance by the global population. The ethno medicinal plant, Murraya koeniggi (Curry-leaf tree) as is native to India exhibits diverse biological activities. Unpublished data from our laboratory revealed hepatoprotective activity of its crude aqueous extract against ethanol-induced hepatotoxicity in experimental animals. Chronic ethanol consumption diminishes the cellular antioxidant levels through free radical induced injury causing hepatitis and cirrhosis with mortality in severe cases. This provided a rationale for studying its mechanistic approaches in terms of modulation of antioxidant defenses for probable hepatoprotective activity against ethanol-induced hepatotoxicity in vitro. Based on the inhibitory concentration (IC(50)) obtained from the cell viability assay, graded concentrations of 100 μg/ml and 500 μg/ml of aqueous extract (WE), isolated carbazole alkaloids (CA) and tannin (T) fraction were chosen to study the hepatoprotective activity against ethanol-induced hepatotoxicity using liver carcinoma cell lines (Hep G(2)). Their antioxidant activity with anti-lipid peroxidation potential (LPO), effects on protein content, liver metabolizing enzymes viz., glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and the morphology of the cells were studied as parameters of hepatoprotection. The tannins and the carbazole alkaloids from the aqueous extract exhibited excellent hepatoprotective activity with respect to the different parameters studied and maintained normal morphology even after ethanolic challenge to the cells as comparable to the protection offered by the standard drug L-ornithine L-aspartate (LOLA). The modulating effect of the aqueous extract and isolates on liver metabolizing enzymes, reduction in lipid peroxidation and decreased cellular damage were found to contribute to the hepatoprotective activity.
药用植物是一种主要的医疗保健资源，这一点佐证了它们正逐渐被全球人口所接受。民族药用植物印度楝（咖喱叶树）原产于印度，具有多种生物活性。我们实验室未发表的数据显示，其粗水提取物对实验动物乙醇诱导的肝毒性具有保肝活性。长期摄入乙醇会通过自由基诱导的损伤降低细胞抗氧化水平，导致肝炎和肝硬化，严重时会导致死亡。这为研究其在体外调节抗氧化防御机制以对抗乙醇诱导的肝毒性的可能保肝活性提供了理论依据。根据细胞活力测定获得的抑制浓度（IC(50)），选择100μg/ml和500μg/ml的分级浓度水提取物（WE）、分离的咔唑生物碱（CA）和单宁（T）组分，以研究其对肝癌细胞系（Hep G(2)）乙醇诱导的肝毒性的保肝活性。研究了它们的抗氧化活性以及抗脂质过氧化潜力（LPO）、对蛋白质含量、肝脏代谢酶（即谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT））的影响以及细胞形态，将这些作为保肝的参数。水提取物中的单宁和咔唑生物碱在所研究的不同参数方面表现出优异的保肝活性，即使在乙醇刺激细胞后仍保持正常形态，与标准药物L-鸟氨酸L-天冬氨酸（LOLA）提供的保护相当。发现水提取物及其分离物对肝脏代谢酶的调节作用、脂质过氧化的减少和细胞损伤的降低有助于保肝活性。
