Iglesias R, Ferreras J M, Arias F J, Muñoz R, Rojo M A, Girbés T
Departamento de Bioquímica, Biología Molecular y Fisiología, Facultad de Ciencias, Universidad de Valladolid, Spain.
Arch Microbiol. 1991;155(4):320-4. doi: 10.1007/BF00243449.
Addition of the L-proline analogue L-azetidine 2-carboxylic acid to growing cultures of Saccharomyces cerevisiae var. ellipsoideus promoted fast deactivation of the general aminoacid permease, measured as L-valine uptake, without an immediate decrease in the growth rate. Cells preincubated with the analogue for 3 h were unable to restore either growth ability or general aminoacid permease activity in analogue-free medium. Eadie-Hofstee plots of L-valine uptake in the presence of the analogue are consistent with a strong reduction in the number of active molecules of the general amino-acid permease located in the plasma membrane. Inhibitory effects on protein synthesis were seen after preincubations of the yeast with the analogue for 3 h although a 30 min preincubation had no effect.
向酿酒酵母椭圆变种的生长培养物中添加L-脯氨酸类似物L-氮杂环丁烷-2-羧酸，可促进一般氨基酸通透酶的快速失活，以L-缬氨酸摄取量衡量，且生长速率无立即下降。用该类似物预孵育3小时的细胞在无类似物的培养基中无法恢复生长能力或一般氨基酸通透酶活性。在有该类似物存在的情况下，L-缬氨酸摄取的伊迪-霍夫斯泰曲线与位于质膜上的一般氨基酸通透酶活性分子数量的大幅减少一致。酵母用该类似物预孵育3小时后可见对蛋白质合成的抑制作用，而预孵育30分钟则无影响。
